FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Kr-h1, Mt2
FlyBase ID
FBig0000123176
Interaction Network
Interactions Browser links
Kr-h1 network
Mt2 network
Reported Interactions
FBrf0258888-1.coIP.WB
Reference
He et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Kr-h1 

Kr-h1

bait

FLAG tag

Mt2 

Mt2

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(He et al., 2024)
FBrf0258888-2.EMSA
Reference
He et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Kr-h1 

Kr-h1

neutral component

FLAG tag

Kr-h1 

Kr-h1

neutral component

FLAG tag

Kr-h1 

Kr-h1

neutral component

FLAG tag

Mt2 

Mt2

neutral component

Myc tag

Mt2 

Mt2

neutral component

Myc tag

Mt2 

Mt2

neutral component

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(He et al., 2024)

S2 Nuclear extracts over-expressing Kr-h1 and Mt2 were mixed with biotin labeled DNA and detected by chemiluminescence. DNA-protein complexes were disrupted by addition of FLAG or Myc antibodies and were used to deduce protein-protein interactions.

(He et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)