Source was fixed S2 cells; proteins produced from transfected constructs; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Bait protein targeted to a co-transfected construct containing a GFP nanobody fused to an ed-FLAG or ed-FLAG-myr cell adhesion protein, which targets the chimera to cell to cell contacts. The ability of this chimera to recruit prey protein to cell contact sites taken as evidence of direct physical interaction.

Bait protein was fused to the ed cell adhesion protein, which targets the chimera to cell to cell contacts. The ability of this chimera to recruit prey protein to cell contact sites taken as evidence of direct physical interaction.

Source was live fixed S2 cells; proteins produced from transfected constructs.

Source was fixed S2 cells; proteins produced from transfected constructs.

Bait protein targeted to a co-transfected construct containing a GFP nanobody fused to an ed-FLAG cell adhesion protein, which targets the chimera to cell to cell contacts. The ability of this chimera to recruit prey protein to cell contact sites taken as evidence of direct physical interaction.