Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

Addition of hydrogen peroxide, Paraquat or Usp7 transfection increased yki-foxo interaction, however this increased interaction was lost in foxo 2KR ubiquitination mutants (K182R and K205R). Additionally, transfection with wild-type N-terminal domain foxo, but not foxo 2KR, diminished foxo C and N-terminal interaction. sd transfection diminished foxo-yki interaction. Additional foxo-HA transfection diminishes foxo-yki interaction.

Addition of hydrogen peroxide or Paraquat increased yki-foxo interaction in a time dependent manner. Usp7 transfection increased yki-foxo interaction.

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Knockdown of sd increased foxo-sd interaction. Addition of hydrogen peroxide increased foxo-yki interaction.