FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Arf1, sqh
FlyBase ID
FBig0000123846
Interaction Network
Interactions Browser links
Arf1 network
sqh network
Reported Interactions
FBrf0262113-1.BiFC.FM
Reference
Gujar et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Arf1 

Arf1

bait

Myc-YFP tag, N-terminal fragment

Arf1 
sqh 

sqh

prey

HA-YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arf1 
interaction domain residue 71
mutation disrupting interaction
aa 71

Q71L; coordinates relative to Arf1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Gujar et al., 2025)
FBrf0262113-2.BiFC.FM
Reference
Gujar et al., 2025
Description
physical association
Assay
Collection
Source/Stage
neuroblast / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arf1 

Arf1

bait

Myc-YFP tag, N-terminal fragment

Arf1 
sqh 

sqh

prey

HA-YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arf1 
interaction domain residue 71
mutation disrupting interaction
aa 71

Q71L; coordinates relative to Arf1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval neuroblasts of transgenic fly line; proteins produced from transfected constructs.

(Gujar et al., 2025)
FBrf0262113-3.PLA
Reference
Gujar et al., 2025
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arf1 

Arf1

neutral component

FLAG tag

Arf1 

Arf1

neutral component

FLAG tag

Arf1 
Arf1 

Arf1

neutral component

FLAG tag

Arf1 
sqh 

sqh

neutral component

Myc tag

sqh 

sqh

neutral component

Myc tag

sqh 

sqh

neutral component

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arf1 
interaction domain residue 71
mutation disrupting interaction
aa 71

Q71L; coordinates relative to Arf1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from endogenous gene; prey produced from transgenic construct.

(Gujar et al., 2025)
External Crossreferences and Linkouts ( 0 )
References (1)