Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and insect cell system; prey produced and labeled by in vitro translation.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was larval eye discs of transgenic fly line; proteins produced from tagged transgenic construct.
