FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Tet, Rel
FlyBase ID
FBig0000123971
Interaction Network
Interactions Browser links
Tet network
Rel network
Reported Interactions
FBrf0265826-1.coIP.WB
Reference
Zhang et al., 2026
Description
physical association
Assay
Collection
Source/Stage
fat body / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tet 

Tet

bait
Rel 

Rel

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fat body of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Zhang et al., 2026)
FBrf0265826-2.coIP.WB
Reference
Zhang et al., 2026
Description
physical association
Assay
Collection
Source/Stage
fat body / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tet 

Tet

bait

FLAG tag

Rel 

Rel

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fat body of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhang et al., 2026)
FBrf0265826-3.coIP.WB
Reference
Zhang et al., 2026
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Tet 

Tet

bait

FLAG tag

Tet 
Rel 

Rel

prey

Myc tag

Rel 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tet 
mid domain
sufficient binding region
aa 865-1560

coordinates relative to Tet-PF

Rel 
N-terminal homology domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zhang et al., 2026)
FBrf0265826-4.PD.WB
Reference
Zhang et al., 2026
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rel 

Rel

bait

GST tag

Rel 
Tet 

Tet

prey

His label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rel 
N-terminal homology domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(Zhang et al., 2026)
FBrf0265826-5.coIP.MS
Reference
Zhang et al., 2026
Description
physical association
Assay
Collection
Source/Stage
fat body / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tet 

Tet

bait
Rel 

Rel

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fat body of wild type fly line; bait and prey produced from endogenous gene.

(Zhang et al., 2026)
External Crossreferences and Linkouts ( 0 )
References (1)