A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{SUPor-P}; insertion stocks created and vetted by the Gene Disruption Project (GDP). The P{SUPor-P} construct carries two visible markers: a w+mC mini-white marker flanked by su(Hw)-binding sites, designed to reduce the variablity of the phenotype of the white gene marker due position effects, in addition to the mini-yellow marker y+mDint2 (FBrf0084323).
Mobilization of the P-element occurred in animals carrying P{SUPor-P} on a second chromosome CyO balancer chromosome and P{Δ2-3}99B, a stable source of P transposase, on a marked third chromosome. Precautions were taken to avoid recovering multiple lines from the same insertion event. ~3000 balanced lines were established.
Insertions were recovered using multiple schemes, of two basic types: (1) mobilization of a P{SUPor-P} construct on a second chromosome CyO balancer chromosome upon being exposed to P{Δ2-3}99B, and (2) mobilization of heterochromatic insertions of P{SUPor-P} upon being exposed to P{Δ2-3}99B. Precautions were taken to avoid recovering multiple lines from the same insertion event.
An aggregate collection of lines produced in the Karpen laboratory using a variety of screens (FBrf0135802,FBrf0149015,FBrf0167658) and by the GDP. Mobilization of the P-element occurred in animals carrying P{SUPor-P} on a second chromosome CyO balancer chromosome and P{Δ2-3}99B2, a stable source of transposase, on a marked third chromosome. The screen was carried out in a y1; ry506 isogenized background known to be free of hobo elements and other sources of background mutations. All X chromosome insertions were kept in this genetic background; most autosomal insertions were rebalanced, introducing different genetic backgrounds.
A total of 10,587 lines were generated for the KG project, including 1236 isolated in initial experiments in the laboratory of Gary Karpen. Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. 2129 KG lines were selected for inclusion in the BDGP Gene Disruption Project 2004 collection.
