Gunaratne, P.H., Mansukhani, A., Lipari, S.E., Liou, H.C., Martindale, D.W., Goldberg, M.L. (1986). Molecular cloning, germ-line transformation, and transcriptional analysis of the zeste locus of Drosophila melanogaster.  Proc. Natl. Acad. Sci. U.S.A. 83(): 701--705.

FBrf0045275

Research paper

Approximately 170 kilobase pairs (kb) of contiguous DNA sequences derived from bands 3A3,4 of the Drosophila melanogaster X chromosome have been isolated by molecular cloning. Sequences required for the wild-type expression of the zeste locus are located within a 6-kb fragment of this chromosomal region, as shown by phenotypic rescue of zeste mutants in P element-mediated germ-line transformation. Expression of zeste is correlated with a 2.2-kb poly(A)+ RNA species transcribed at all postzygotic stages of Drosophila development. Many zeste alleles, including several producing neomorphic phenotypes, are not associated with detectable rearrangements of DNA.

PMC322932 (PMC) (EuropePMC)