Delgado-Rodriguez, A., Ortiz-Marttelo, R., Graf, U., Villalobos-Pietrini, R., Gomez-Arroyo, S. (1995). Genotoxic activity of environmentally important polycyclic aromatic hydrocarbons and their nitro derivatives in the wing spot test of Drosophila melanogaster.  Mutat. Res. 341(4): 235--247.

FBrf0079981

Research paper

The genotoxicity of three polycyclic aromatic hydrocarbons (PAHs) and of three of their nitro derivatives was evaluated in the wing Somatic Mutation And Recombination Test (SMART) in Drosophila melanogaster. Two crosses were used, i.e. the standard cross (ST) and the improved high bioactivation cross (HB) which is characterised by an increased sensitivity to the genotoxic effects of promutagens and procarcinogens. Larvae trans-heterozygous for the two recessive wing cell markers multiple wing hairs (mwh) and flare (flr3) were fed with the test compounds for 48 h. The wings of the surviving flies were analysed for the occurrence of single and twin spots. Naphthalene, 1-nitronaphthalene and 1,5-dinitronaphthalene proved to be more genotoxic in the HB cross than in the ST cross. Anthracene showed a clear genotoxic activity only in the HB cross whereas it was negative in the ST cross. 9-Nitroanthracene gave inconsistent results in both crosses. Phenanthrene was negative in the ST cross, but weakly positive in the HB cross. These results demonstrate that the genotoxic activity of these PAHs and their nitro derivatives can be detected with the somatic cells of the wing imaginal discs of larvae with high bioactivation capacity.