Dear Chihiro,
Indeed, mákos (mks) corresponds to cdc27. This gene was previously
identified at cDNA level only. Now, mks has two mutant alleles, mks1
and mks2. mks1 was identified from our P collection and its
identifier is l(3)092309. mks2 is a P-lacW insertion allele as mks1
but it comes from the BDGP and its available from Bloomington. The
identifier number of mks2 is l(3)L7123 and the stock number is P168.
The plasmid rescue sequences from these lines overlap with each other
and with the incomplete cDNA sequence (U18298) in the database. The
insertion has been localized to the 5'-UTR in both alleles and they are
about 250 bp apart from each other, mks2 being closer to the 5'-end of
the transcript.
mks1 is recessive pharate adult lethal
mks2 is recessive semilethal and female sterile
In double mutant combinations, they show genetic interaction with
several mitotic mutations, such as tws(or aar), polo, string, asp and
bub1.
There is a noticeable discrepancy between the localization of mks1 and
l(3)L7123. I mapped mks1 to 65E7-12 but according to FlyBase,
l(3)L7123 was mapped to 65F1-2. Because of that, I double checked my
preparations and photos and I'm quite sure that the insertion is in 65E
(the in situ signal and the 65F1-2 double band appear separated on well
spread chromosomes and the signal is distal from 65F1-2.
Cheers,
Peter
Hiya Peter,
We are currently curating the abstracts for the upcoming European
Drosophila Research Conference in Zurich, for FlyBase. I am writing in
connection with your abstract:
'Characterization of makos, a new mitotic gene encoding a subunit of
the anaphase promoting complex in Drosophila melanogaster'
Could you confirm that makos is the same as the gene in 'Cdc27' gene we already have in FlyBase (accession numbers: U18298, Q24021). I'm pretty sure they are the same, but just wanted to check.
Also, what is the identifier number of the P-element mutation in makos
\- is it one of the Szeged lines from your paper Deak et al., 1997,
Genetics 147(4): 1697--1722?
Thank you for your help,
with best wishes,
Chihiro