Subject: Ercc1 and mus210
I recently submitted a Genbank entry, AF146797, for the Drosophila gene
encoding the homolog of the human nucleotide excision repair protein Ercc1.
I had concluded that this protein is the product of the mus210 gene on the
basis of:
1) The Ercc1 coding region maps to 51D1-2 by probing the P1 blot.
2) Hypersensitivity of mus210 mutants is uncovered by Df(2R)Jp1 (published
breakpoints 51C3; 52F8-9 and Df(2R)XTE-18 (51D3-E1;52D1), but not by
Df(2R)Jp4 (51F13;52F8-9).
3) The mus210B1 chromosome has a single nucleotide change (relative to
other wild-type chromosomes) that changes a conserved Thr to Ala.
I have since discovered that mus210 does not correspond to Ercc1. I was
able to obtain a stock of an unrelated mus mutation that came out of the
same screen as mus210B1, and discovered that the Ercc1 alteration in
mus210B1 is a pre-existing polymorphism. I also learned that Df(2R)Jp1
and Df(2R)XTE-18 do not delete Ercc1. Df(2R)knSA3, however, does delete
Ercc1, but does not uncover mus210. Complementation crosses with lethal P
element inserts mapped by Todd Laverty suggests revisions for these Df
breakpoints:
Df(2R)knSA3 uncovers l(2)k08015 (51D3-5)
Df(2R)knSA3 uncovers l(2)k06403 (51D7-8)
Df(2R)knSA3 does not uncover boc (listed as 51D, but on a sequenced contig
it is distal to chn, which is in 51E2)
Thus, the distal breakpoint for Df(2R)knSA3 is probably 51D7-E2.
Df(2R)Jp1 does not uncover l(2)k08015 (51D3-5)
Df(2R)Jp1 uncovers l(2)k06403 (51D7-8)
Thus, the proximal breakpoint for Df(2R)Jp1 is probably 51D3-8.
Ercc1 maps to 51D1-2, is uncovered by Df(2R)knSA3, but not by Df(2R)Jp1 or
Df(2R)XTE-18. There are no known mutations in this gene. I've sent an
update request to Genbank to have them change the name associated with
AF146797 from mus210 to Ercc1.
So what is mus210? I've found that it corresponds to Xpcc, which encodes
the nucleotide excision repair protein XPC, and which maps to 51F. The two
published alleles are mus210B1 and mus210G1, formerly mus(2)201G1 or
rad202G1. This latter allele is incorrectly listed in Flybase as an
allele of mus201 (FBal0012621). Both alleles are nonsense mutations in
Xpcc, and I've recovered two additional alleles (C1 and C2), which are also
nonsense mutations (manuscript in prep).
Jeff Sekelsky
Subject: Re: Ercc1 and mus210
>1) The Ercc1 coding region maps to 51D1-2 by probing the P1 blot.
A ha - Jeff if you can tell me which particular P1 clone Ercc1 maps to
I can usefully capture that info for FlyBase, too.
Thanks very much,
Rachel.
Subject: Re: Ercc1 and mus210
Hi Rachel. Ercc1 hybridizes to:
DS09161 (51D1-2 by in situ)
DS06422 (positive for Dm0765 from DS09161)
DS06145 (positive for Dm0764 from DS09161; this STS was not used in contig
building because of some ambiguous results, but clearly this P1 belongs on
this contig).
It does not hybridize to DS00158, which is also positive for Dm0765 and
which has been placed in 51C1-D2 by in situ.
So, Ercc1 is in the region of overlap between 09161, 06422, and 06145.