Dear FlyBase,
My name is Toby Wu and I am an undergraduate working in Dr. Celeste Berg's
laboratory at the University of Washington. Two years ago, I sequenced two
alleles of Drosophila ras, Ras85DSutor-341 and Ras85DSutor-404, as part of
my project. I have included the molecular data for these two alleles
below.
Ras85DSutor-341 consists of a nonsense mutation that affects amino acid
\#131 (Arg to Stop). The insertion of a new stop codon 3bp upstream of the
second intron results in truncation of the protein, thereby eliminating
the CAAX box.
Ras85DSutor-404 consists of a 5' splice site point mutation in intron 1,
which changes the conserved GU to AU. The altered 5' splice site may no
longer serve as a docking site for the U1snRNP complex of the spliceosome,
and subsequently lead to incomplete RNA splicing.
At your earliest convenience, please include this Molecular data into the
FlyBase Report of these alleles.
Thanks
Toby