5' splice site point mutation in intron 1, which changes the conserved GU to AU. The altered 5' splice site may no longer serve as a docking site for the U1snRNP complex of the spliceosome, and subsequently lead to incomplete RNA splicing.
Ras85DSutor-404/Ras85D05703 transheterozygotes have low viability. Females transheterozygotes with Ras85D05703 lay eggs with a severely disrupted eggshell (patch of chorion instead of dorsal appendages), 25% of eggs hatch and develop to normal larvae. Transheterozygotes with Ras85D05703 exhibit slightly disrupted ommatidial array (small number of fused and misplaced ommatidia).