Subject: P{UAS-shi.K44A} insertions
From: Kevin Cook, Bloomington Stock Center
Subject: P{UAS-shi.K44A} insertions
The following information came from Amy Bejsovec, Northwestern University
(5/3/00).
P{w+mC=UAS-shi.K44A}3-10 is an insertion on a Sb+ TM3 balancer
chromosome. When expressed using a uniform Gal4 driver, it provides a very
high level of endocytotic disruption, comparable to the original shits1
mutation. It phenocopies the cell-lethal effect of shits1.
P{w+mC=UAS-shi.K44A}4-1 is a homozygous/hemizygous viable and fertile X
chromosome insertion. P{w+mC=UAS-shi.K44A}3-7 is a homozygous viable and
fertile second chromosome insertion associated with a recessive curled wing
phenotype. Together these insertions provide a moderate level of
endocytotic disruption when expressed using a uniform Gal4 driver line.
Separately they each provide a low level of disruption.
The construction and transformation of P{UAS-shi.K44A} was described in
Moline, M. M., Southern, C. and Bejsovec, A. (1999). Directionality of
Wingless protein transport is essential for wild-type epidermal patterning
in the Drosophila embryo. Development 126, 4375-4384 (FBrf0111433).