Subject: Helping FlyBase: srGAL4
Dear Vijay,
I am curating this paper of yours, for FlyBase:
\*x FBrf0131298 == Ghazi et al., 2000, Development 127(24): 5309--5318
I have a couple of questions, about the srGAL4 and the UAS-GFP you
use.
We have no record of an srGAL4 in FlyBase so I wondering whether you
would be able to tell me whether this is an enhancer trap type
insertion of P{GawB}? (If it is from the big Madrid screen then do you
know the identifier number for it? We may already have a record of
it, but not yet been told that the insertion falls in sr).
Alternatively it could be a promoter fusion construct, in which case
FlyBase would store the data in a different format \- which is why I ask
for the clarification.
Also, we have several UAS-GFP constructs. Which UAS-GFP is the one
recombined onto the srGAL4 chromosome? If you could give me either a
Bloomington stock number, or a reference to a paper where it has
previously been used then I would be able to figure it out.
Many thanks for your help,
With best wishes,
Rachel.
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Rachel Drysdale, Ph.D.
FlyBase (Cambridge),
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Subject: Helping FlyBase: srGAL4
Dear Dr. Drysdale,
Thank you very much for your mail and for curating our publication for
flybase. In answer to your queries,
\-Yes, srGAL4 is from the big morata/madrid screen. Identification number
is md710.
\-However, the construct we've used in our paper is a RECOMBINANT OF
'SRGAL4 MD710' WITH UAS-GFP ON THE THIRD CHROMOSOME and NOT md710 in its
original form.
\-This recombinant was generated by Daniela Pistillo is the laboratory of
Pat Simpson <up>now at Cambridge</up> and was given to us before
publication. This is the reason you could not find it described anywhere.
\-I'm afraid I DO NOT know the nature of the UAS-GFP used for the
recombination but can ask Daniela if you think the information is crucial.
I do hope I've been able to answer your questions satisfactorily. Please
do let me know if I can be of any more help. Thanks once again for your
efforts with our publication.
Best regards,
Arjumand