Subject: Isolation and Characterization of Df(2L)BSC4
Isolation and Characterization of Df(2L)BSC4
Jennifer Deal and Kevin Cook
Bloomington Stock Center, Indiana University
Df(2L)BSC4 was isolated as a P transposase-induced male recombination event
involving P{lacW}Rpp30k01901 and P{PZ}alpha-Adaptin06694. The deletion
was isolated as a net-cn recombinant chromosome from the cross net1 b1
cn1 sp1 females x net1 P{lacW}Rpp30k01901/P{PZ}alpha-Adaptin06694
cn1; TMS, delta2-3 males. The Df(2L)BSC4 chromosome retains the miniwhite
marker from the P{lacW} construct. Polytene chromosome squashes showed the
breakpoints 21B7-C1;21C2-3. Df(2L)BSC4 failed to complement
P{lacW}RpI135k16513 and P{lacW}U2af38k14504, Hopk14504 (a single
P{lacW} insertion affecting adjacent genes).