Subject: Novel kinase: STLK
Genomic sequence analysis predicts a novel fly kinase: Stlk (Ste20-like
kinase) is a member of the Ste20 (MAP4K) family of kinases that also
includes Drosophila genes msn, mbt, ninaC and DPak, and is most similar to
STLK5 and STLK6, two yet-to-be-published human kinases, and the Y52D3.1
gene of C. elegans.
The peptide sequence has been published in TrEMBL (accession P83098). It
was created by a HMM search of the genome sequence followed by gene
extension using genewise with the human STLKs as templates. The sequence is
partially confirmed by 10 ESTs: 9 from embryonic sources (7 from the LD
library, 2 from RE) and one from ovary (GM library). The peptide reading
frame is open at the C-terminus and probably incomplete; two of the ESTs
have mate pairs (LD05623.3prime and LD01213.3prime) which map to the
genomic sequence starting 1.5 kb downstream of the end of the peptide and
probably are part of the 3' UTR of the gene. These sequences may be useful
in designing RT-PCR primers to find the C terminus of the gene. The gene
has three exons and maps to 41F1.
\-Gerard Manning.