Subject: P{ey-FLP.B} construct and insertion
The following information was provided to the Bloomington Stock Center by
Michael Brodsky, UMass Medical School (5/02).
He described the construction of P{ey-FLP.B} as follows:
'My ey-FLP construct was generated by cloning the 0.2kb eyeless enhancer
fragment described by Halder and Gehring into the pHZ50pL enhancer
detection vector (at the XbaI and KpnI sites) and then replacing the
TATA-box and lacZ gene (cutting at KpnI, SalI, and blunting) with a
fragment containing the minimal hsp70 promoter and FLP gene from Golic's
pDM420 derivative (from the NruI site in hsp70 promoter and XbaI site
adjacent to pDM420).'
P{ey-FLP.B}3.1 is an insertion into a Sb1-marked TM3 <up>most likely
TM3-rSS, K.C.</up>. This balancer variant will be denoted by the balancer
short name TM3, P{ey-FLP.B}3.1, Sb1.