Subject: methuselah family of GPCRs
Dear Gillian,
..
for a small part of a manuscript we have almost accepted in Science
for the A. gambiae genome issue this Fall I have worked up the methuselah
family of GPCrs. I see that West et al named the genes last year in PNAS in a
mth-like series, abbreviated mthl in my work ..
Anyway, the annotations of these proteins remain somewhat poor in the
public databases, so I'm not sure if they have been updated to what they
published. Any case, I've also added two members, 13 and 14, by fixing the
annotation of GH30018 and recognizing RE31310p as a family member,
respectively. Below is a simple FASTA compilation of the resultant family.
I've done this pretty carefully with detailed alignments of intron positions,
etc, and am pretty confident of them from a bioinformatic perspective, and
several now have full-length cDNAs, but if you have questions about any of
them please let me know.
Hugh
>mth
MKTLLVLRISTVILVVLVIQKSYADILECDYFDTVDISAAQKLQNGSYLFEGLLVPAILTGEYDFRILPDDSKQKVAR
HIRGCVCKLKPCVRFCCPHDHIMDNGVCYDNMSDEELAELDPFLNVTLDDGSVSRRHFKNELIVQWDLPMPCDGMFYL
DNREEQDKYTLFENGTFFRHFDRVTLRKREYCLQHLTFADGNATSIRIAPHNCLIVPSITGQTVVMISSLICMVLTIA
VYLFVKKLQNLHGKCFICYMVCLFMGYLFLLLDLWQISISFCKPAGFLGYFFVMAAFFWLSVISLHLWNTFRGSSHKA
NRFLFEHRFLAYNTYAWGMAVVLTGITVLADNIVENQDWNPRVGHEGHCWIYTQAWSAMLYFYGPMVFLIAFNITMFI
LTAKRILGVKKDIQNFAHRQERKQKLNSDKQTYTFFLRLFIIMGLSWSLEIGSYFSQSNQTWANVFLVADYLNWSQGI
IIFILFVLKRSTWRLLQESIRGEGEEVNNSEEEISLENTTTRNVLL
>mthl1
MDRSRSSRAASSNQFIRPCGLLTTVILLQTLVSMSLAIEEMSPPPAAPPRPSPPPTVKLNKCCHSGEYLNDGTCIAGS
EALWLPMVYLVQQQRFFEPHGASPRFLKFLPNTRPTCRKDQTTEIFRSRGANVMLFPNGTLYVRERALMVQPSDYCVD
WEVAVVCLNDSQPINALEDPDYAANPLVQQEPPKASLRLSKCCGKWGSYNTQLQNCDLQPNHQAAVDGLLRLSPQLPE
GSYQTSYGLPDCGQPGGYSIAGDWQDAKLDRNTAMLQLPHKNLSAGQYCLEHTQREGEVKIIACQHLFSSAAGAGIHD
GSIGGTIEQANGQNLQKAVLTGGILVSIVFLSATLVAGFLLPAVHHALHWRCQICYVTCLLFGKILLAIEELSSSLQP
GSAACHTLAITMQFFFLAAFFWLNTMCFNIWWTFRDFRPSSLERNQEALRRYLYSLYAWGGPLLITFVAACVDQLPET
TLLRPGFGQLYCWFDNRNLSIFAYFYGPIGLLLCANIALFVSTTHQLTCGLWKRDDVKSSSEKSALGRVCLKLVVVMG
VTWIADILSWLVGGPHGVWFFTDLINALQGVFIFIVVGCQPQVWTACRRIFCPRLRHDITNTTNGVQHSSSSQGLPSM
AGGTEITQNTTTTTTTTNTTATHMPSNPAEDEVPEKAPIAPVAPIVKMETIC
>mthl2
MLLSASILIYFLLNLQSSSAEIADCSFYDTVDISEGQRLSNGSYLYEGLLIPAHLTAKYEFKLLANGDKEQVPSHVRG
CVCKLRTCVRFCCPHDHIMDMGECYANMTTEENELLDPMLNVTLDDGSVVQRHYKKELMVQWDLPKPCDDMFYLDNRD
IMDEYTLFENGRLLRHYDQVYLDKSEYCLQHRTFGEGNNNSIRIIPHNCLILPSRTGQTVVMITSLICLVLTIAVYLC
VKKLMNLEGKCFICYMMCLFFGYLFLLLDLWELSLDFCKAAGFLGYFFVMAAFFWLSIISRHYWKCLTNPCASMNIRS
ERAFLLYSCFAWAMPLALTGVTYLADNVVNNEEWQPRVGDEGHCWIYTKSWSAMVYFYGPMVLLILFNITMFVLTAKH
IIDSKRTLRKIARNEGRIQKLNSDKQNYTQFLLLFTVMGMSWSFEIFSYLVQREKLWVNIFLVADYFNWSQGVIIFVL
FILRRKTLVLFKKQIFPKQRAFSRSATQSTIESISQTKRHFNMT
>mthl3
MRIVIGSFTAFLLLLLQNSNAEIPGCDFFDTVDISKAPRFSNGSYLYEGLLIPAHLTAEYDYKLLADDSKEKVASHVR
GCACHLRPCIRFCCPQYQKMQKSKCYGDMSEDELNKHDPFVNVTLSDGSVVRRHFKEDLIVQSDLAKPGCPRMYFLNH
ELPGNEFTLFENGSLLRHWDKVELSKREYCVQHLSFKDDSIRIAPHFCPLSSEHSRTWKTVAIVISLICIILTISVYL
YVEKLRNLHGKCFICYLASLFLGYFFLVLNVWKYSSGFCVTAGFLGYFSVIAAFFWLSVISLTLWNSFSGNSSWLNRF
LPQNRFLSYNLYAWGMALLLTAITYIADQVVKNEKLRPRVGVGKNCWIYTGDMTVMIYFYGPMLLIIVFNITMFVLTA
FRIMKVKKEAQNFTQQQKTTNRLNSDKQTYALFLRLFIIMGLSWSLEIISFLLSKNQAWAKAFMVADYFNWSQGTVIF
LLFVLRPSTLKLLKERIKGGRDEAGASDEHISLQNTKIDPSVF
>mthl4
MRILLIAVLFLLMPKSNAEIPGCDFFDTVDISKAPRFSNGSYLYEGLLIPAHLTAEYDYKLLADDSKEKVASHVRGCA
CHLRPCIRFCCPQYQKMQKSKCYGDMSEDELNKHDPFVNVTLSDGSVVRRHFKEDLIVQSDLAKPGCPRMYFLNHELP
GNEFTLFENGSLLRHWDKVELSKREYCVQHLSFKDDSIRIAPHFCPLSSEHSRTWKTVAIVISLICIILTISVYLYVE
KLRNLHGKCFICYLASLFLGYFFLVLNVWKYSSGFCVTAGFLGYFSVMAAFFWLSVIGIHLRIKFSLASNCLHRLLPE
NPFRAYNLYAWGIPLIMTAITYTADQVVKNEKLRPRVGVGKNCWIYTGDMTVMIYFYGPMLLLIAFNIIMFVLSAIYI
YNIKKNVKGLVHKQQTNQQINDQQMFAIFLRLFILMGLSWSFEILSFLLTKQQAWARALMVADYFNWSQGTIIFVLFI
LKPSILKLIIAGGRQNLPGSHHNSRSKAARYNSTHTACEGSIADPNAYC
>mthl5
MLVKTLGAHFAAGQNAKKCSCCALLISLLCVLLLSLNPLPVTSHVTSAGSSTALSSDPNLVLFNKCCEKFEIHVDHEC
QQVNETDYFQPMFTSYGGEQNRPVKFKFVIGIPNCGSMQMWPIYHYAGSSDKLVLLDDGRLRHYTNAENEAEERHGIQ
SDYEEDIAGSLEPLYHDYDKGLYCIDKATSSTGEENVLFANICLARKEIKWSDSNFLLRKILNPIFHGISLVILLVIA
IIYFILPTLRDLVGNIVTTIAMCLMVSQAADLVRIFTELTSHVSFIVADIILCFSLLAAFFWLNSFGFYIWKTFRSRN
VFLRVTDGRKYCYYSAYAWGCTATMAALAVFAHFFLDAESYKQEHMVGEQETIGWLGICIFFAPIACTILVNIFFYVT
TRKLINRRTVYGRIAHKLKANFIMFSLMLLVMSIAWLFLIMSWLQMEGLLYAHIVVNALQTPLLLYICVLRQRHVTFL
LKKTCCYNEPPSANDWGDELHYMNGNDY
>mthl6
MLLNILAIILVFVISSQSEAVIPGCDYFDTVDISHIPKLNDSYAYEELIIPAHLTGLYTFRQLADGSQEPVKSHLRAC
ICKLKPCIRFCCPRNKMMPNSRCSDGLTENLKRINPYLKITLEDGTIGKYYLLTDMIVLRYEFRYCEKVVSVQEDQYK
LYENGSFMIKPDVNWTLSKQWYCLHPRLEDPNSIWILEHVYIPKSMPAVPQVGTISMVGCILTIAVYLYIKKLRNLLG
KCFICYVFCKFVQYLIWAGGDLNLWNNICSLAGYTNYFFALASHFWLSVMSHQIWKNLRLINRDERSYHFLIYNIYGW
GTPAIMTAITYLVDWAWEDRPDKLNWIPGVGLYRCWINTYDWSAMIYLYGPMLILSLFNVVTFILTVNHIMKIKSSVK
SSTQQQRKCIQNNEFLLYLRLSVMMGVTGISEVITYFVKRHKFWRQVLRVPNFFHLGSGIVVFVLFILKRSTFQMIME
RISGPRRQQPAS
>mthl7
MRLPWVIFCTVLLLIFTNNSNADIPGCNYYDTVDISYIERQNDSYLYDDIEIPASLTGYYEFRQFGDGSITPIEKHLR
ACVCSVRPCIRICCPAKNFLANGKCDDGLKEELARFKPYIYFTYMDLQARVPLTDMAIIRDEFFDCDEMIYISDFNYF
LEEDGKFWVTVDLFMEKQDYCLYRHNFDSDFPKSMWIIRHRCTSHISPGSLEILIITMICFVLTIAVYLYIKKLRNVT
GKCIVCCIVSRFIQCLIMILDHLNLLNGICSPAGYSSHFFRMASNLWLSVISYHTWKVLTSLNRVDPNYRFLRYNAFV
WSTAAIMTGSIYIVNQIWENDPSKWNWLPLVGFIRCSVKDWHPSVWIYISGPSLALSTFNVAMFALTAIYIRKVKGGI
NKFTNEEEGRINCINFDSQTYLQFLRLSIVMGLTWIFNVIPYSARLHIFWEWVGIISEYFHSAFGIVLFVLLVLKRST
WTLMMDS
>mthl8
MAQFCILGVLLILSGTHCSWGFHEETHYPCAFIDTANITGSYGLDGPFVHNWTVIPRHFVAVYDFVIENGIRIPASRH
LRACVCKTKPCVRICCLRGEIYDLEKRQCLVPVAGVSSLPSHSHMEVELGNGSLRLVKLQPRFSIHVETPCEHMKAVT
KGSEYVHWTLHENGTISHRGHIFSKHYCFTPLLHGNSTWEWQPLACAPEKLYFVLGVREWTYAICLLIAILSMFIVLM
VYLMCSEMRNSFYGVAIKAYAICMILGYALLAYLTLHNPANLSNAACRILPSLALMNLVLSFYILSFIAFKLYLSFYG
VVFTKLMFWLIFTPIVLVAVGWSFFVGFSYYGSRLIFGGDTCWFDPRNWSVMIYFYAPVFVACAISGFFYVLSQIYIR
DQPDIETEKSFESIEKNRFKSFWKYFGYTAVVWVVCICSFAFNYYWENRSHLNYAVSFCMAFHGFAALYALIGKNQQI
QNFLRRIDNGEDTCENSVPLSSFG
>mthl9
MVSPLIILLIIWLSVGAKSVEIASINHPCAYAHTVNITDGLRMKDGSYSYAGVVVPPHLMAEYSFKVIDGVEYRAKKH
LRGCVCLLKPCISFCCPENLVFDAKHWNCTMPHQVRESTHVELTYANRTVDQVRIRDRFVVRTELGCRNKFVDKKHDN
FWQWDLFENGTLRRDNRLWSTDEYCFSPLEHNPEQWELTPLNCERFQTGYRVWIYAICSIIAIIINIFILSLLGSVRD
ARKSHYGQLIIYYLLSMIVGYSLLVYLALKNPMKLSHVACRNIGFLAYFCIMLSFVFLAICSLDFLLKFKQKAVRSSV
RRLSLALAVLAVIGLRFLVSLAQDSKLPKHFKPGMGEDYCWFDVRTWGILIYYYGPIALLLIFSIVCCLKAYFSIYEL
PPDTQYILGTQLKIVKTHFYAFSAYIVGVFAVWIREIVVYIMARVREHFFIIDFWSGICILGLAIAGFILLLGKNLHV
KSWWAINVESSQTDLSIINARVYKFDEKGDLKSSDSPYKPTVTSL
>mthl10
MPKKIHQPGGSLYCGVTLLGVLCLVVFRLIPGIPFGTYVMAERDHYHTIDDPNVPCNFYDTVNLTGHRLFPNGSYDYY
GTIVPAELVGTYDYIHSSLTERIEVREHVRGCVCKFKSCLNICCPWRQVFNSEVDGCIIDHSDNRTWPDPPMLNITFR
NESTILVNMFTQFAIQSFRPCPKMFSLQPETNNWDDYLLFENGSMLRVDDKLLIRKNEFCMVPTYVNESDMFYTIHPA
NCDMQDDHSTVKIINSYAMMFSIPFMMLTIAVYLLIPELRNQHGKSLVCYLIGLSVGYSSLCYVQLYQVDATGVTCKV
FGYTAYFFFMGAYMWLSVISFDLWHNFRGTRGINRFQEKKRFLFYSLYSWGIALVFLAFTYCAQQLTNLPANLKPGIG
DGVYCWLDMSNWAAMIYFYGPILAIVVANTIMFIMTAIKIHGVQREMARIIASENSTKNLRTEKDKFGLFLRLFLIMG
ITWLTELISYFVGSDKGWSKLFYISDLANAMQGFLIFMLFVMKKKVKHLITNRCSSVRDGSNQRQSQYSTKTTSSSVA
NLSLHEKPSVEKPLVISSSVDPQKTTIFR
>mthl11
MGMFRVEYLLLGILVIGVRSRDIPNCDFFDTVQLRESEKLCNGSYRYEDVVIPAKLTGKYDYEIDYDGDRVSVPKHIR
GCVCKLKTCIRFCCHHKKLMAGNLCSQDVYENLTYEYTLDITQLNGSVIKKHVLNDMVVQQDLPLPCERHYSLDAETS
TYDMWSLYENGSLFRHFDQRYLSKQEFCLQPNPTSTGKNYSLIVAFNCIQKPSMKMAYVKFSSVFFMVITIAAYLWLP
KFRSLHGKCCNLYFICLAITFLLNVISLFGIFELKTPICYLTGYAGYFTVMATFLWLSVISFDVWRRFAMRKFQVFYK
NKRSSFFNYNIIVWSSAGLLTCIIFLVDQFVETNLDNPYNPAVGVFSCWIFTNGWSATFYFYAPLAILIILNCASFFL
TTRYIYVENKQNQKVLNNSEPQKLSRNHANYRIYFRLFIIMGGSWFLEIIAFICEMENMWKPLIILNDYINCSQGIII
FVATFCNHEMFRLIRKRIQNRNITSLELTNTSRPVESEKMADVELGK
>mthl12
MFLWLKCFCTLIIVTIAKNSSAKIPHCKYDETINISHFKRLNDAYIYEHFEIPANLTGEFDYKELMDGSKVPTEFPNL
RGCICKVRPCIRICCARKNILSNGECSDGVKNEIKLTMLDLTMQDILLTDPTLAELNMIPQYNSTELLILREQFQPCD
EIVSLKRDEYTIFKDGSILLHTSAEILSNDQYCLYPEIYSDFPETIRIINRRCYRNVMPGIAQLSVISVVGFILTLAV
YLSVEKLRNLLGKCLICSLFSMFMEYFIWTMDYFRLLQSICSAAGYMKYFFSMSSYLWFSVVSFHLWELFTSLNRHEP
QYRFLIYNTFVWCTAAIPTVVIFSMNQMWENDPGKSEWLPLVGYFGCSVKDWNSSSWFYSHIPIVILNSFNVIMFVLT
AIYIWKVKKGVKSFAQHDERNTTCLEFNVQTYIQFVRLFLIMGASWLLDQLTRLAEDSHLLLDTIVLNLTVYLNAAFG
ILIFVLLILKGSTFKMIMERIRGTRFMTCLKGNQ
>mthl13
MKLQLLGTFLVLILLQFQAVNTDSNKTEEDSCVKSDDDNSNDEDDLWHCIPICCPRKNMMANGGCSFKEHLFRTKLDL
NIRLDDNSTEALYFNNQTLLITSFWDIDEMMGLRRDEYTLYKNGTIYIHSDQSIQTKEEYCFYPHQIYSDFPETIWII
SHKFSSIESPGAFELASGSIICYIIIFGIYLFVKELRNDFGKCVMSCVFCLFLDYLIWLMDELRLLDDFCSLTGYIMF
FFDFANSVWFSIISYCIWKKITSVVSQENRDQFVFYSTFAYGISAIPLGIIISINQFWEEDLRKWNWLPLVGFYLLQF
SDCKRSSWVYYFVPYAIMCAINIIMFVLTIKHIMKTKRNLHNLTKRPDRNETCVTVNFLNFELYLLFLRISGIMGVAW
ILIIFLLIEVNSTFWDIFGIIIQQIHYGFGIILFVLLIFKRSTHQLLIAKIRGKNLHQQTDV
>mthl14
MNLGHWNFLLALISLQTFFNASAQISTVNNSSKGSNNSNIFHEDTFNSTSIDVLDASIHSTLVVPQTYSTEAATISGA
RFATSVPVQSPVDNPLDPADCSQREKYRKQPVATPSSRLRKCCPHGENLNIYRENQSDSMCDNGLLSFEPTIISAVLF
DNCIEDLEVETTLDYDIGNPCNSSLLYDDKDDVFFVLQDGSLLIIDKFGNESYTVKEHYCLDIDKSGHLFAFTCVTQV
EEQIAFAKVVFVAVLMLISMPCLLLVSYLHMTLRLLRNLHGLSLSLMSLCLASGYFVHSVVHIYGIPNQGFIGYVIQF
CILSYFFWYLCICFNVLLNVWYKLPCCIQCSKSWATFNFACYAVFAFSGPATIVALTVQKGLPGMPSYFLQGLTESIR
DSQRYFIPPVSTILFLSFLLNIISFFGFQRISGYAKAEKNIQERKCLFDQQKYEDVKKDAKCVSLLGIIMVVSWLLEI
ITFYSGSNSNYLILCDMVNGLQGVWVLLIFLVVRRRRTIILRWWYDRGSHEIEGTELQALSNSPT
Hugh M. Robertson, Professor
Department of Entomology, University of Illinois at Urbana-Champaign
320 Morrill Hall, 505 S. Goodwin Ave., Urbana, IL 61801