Subject: FlyBase query: Gottingen P-element lines Dear Ulrich, I am doing a final check on my curation of the supplementary information from your paper Peter et al., 2002, EMBO Rpts 3(1): 34--38 about the Gottingen lines, and I realise I forgot to ask you about one particular line \- sorry. The line in question is: l(1)G0462,AJ426758,,7C8-D1,7D1-3,CG2206,L1-L2 so the data from the EMBO Rpts info suggests that this line is a single insert line, with the insert mapping to 7C-7D. However, we have a personal communication from you from 14th December 1999, in which you stated that this line was a multiple insert line, it is FBrf0125056. here is relevant bit of the pc: Personal communication from: Ulrich Schaefer, Herbert Jackle, Yuchun He, Hugo Bellen, Todd Laverty and Gerry Rubin To: Bloomington Drosophila Stock Center Subject: Goettingen lethals \- set 6 Dated: 14 December 1999 New Alleles (presumed to be caused by P{lacW} insertions): l(1)G0462G0462 ? \-- multi-insert line: 3A, 10E-F New Transposon insertions (from multi-insert lines): P{lacW}G0462a 3A P{lacW}G0462b 10E-F can I assume that the more recent data is correct and that you assume that there is actually only one insert in this line at 7C-7D ? sorry I forgot to ask you before, Gillian \-------------------------------------------------------------- Gillian Millburn. FlyBase (Cambridge), \-------------------------------------------------------------- Subject: Re: FlyBase query: Gottingen P-element lines Dear Gillian, line l(1)G0462 is mapped by in situ to 7D1-3 and thus in accord with the molecular data. The only line I have that is mapped by in situ to 3a & 10E-F is line l(1)G0466. The best explanation for the FBrf0125056 note is, therefore, that a typo \- .. \- caused the error. By the way, the latter line is one of the more than ten lines where we could never substantiate the second in situ site at 3A whereas the other \- variable \- site was always verified molecularly. Hence, we are quite confident that the second 3A site is an artifact. .. Ulrich \-- Dr. Ulrich Schaefer Max-Planck-Institut fuer biophysikalische Chemie Abt. Molekulare Entwicklungsbiologie 37070 Goettingen GERMANY Street address: Am Fassberg 11, 37077 Goettingen
