From: Rachel Drysdale (Genetics)
Sent: 3/2/2005  7:52  AM
Subject: Helping FlyBase: ADRC-50399
Dear Cindy,
We are currently curating the abstracts for the upcoming 46th
(San Diego) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
Fratboy, a Dynamin related protein, affects mitochondrial morphology and
transport and is required for mobilization of the reserve pool at the
synapse.
You mention a gene symbol that is new to FlyBase, fratboy. Do you know
which of the Genome Project CG annotations your gene corresponds to?
All the CGs have corresponding gene records in FlyBase already and we
don't like to make duplicate records for what is actually the same gene
unless we can't avoid it. The CG symbols become synonyms when an
annotation is named with a more descriptive or functional name.
Also, if you have settled on a short symbol for your gene now would be
a good time to let me know what it is, as then I can enter it in the
database from the outset.
With best wishes,
Rachel.
\----------------------------------------------------------------------
Rachel Drysdale, Ph.D.
FlyBase (Cambridge),
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Subject: RE: Helping FlyBase: ADRC-50399
Dear Rachel,
We isolated 2 alleles of the fratboy (frat) complementation group
(Verstreken et. al., 2005) in a forward genetic screen for defects in
neurotransmitter release (Koh et. al., 2004; Verstreken et. al., 2005).
Both frat1 and frat2 were mapped to the 22F-23A interval by recombination
(Zhai et. al., 2004) and fail to complement Df(2L)C144, Df(2L)dppd14, and
Df(2L)Exel6008 (Parks et. al., 2004). Additionally, the frat alleles fail
to complement l(2)22FdT26, l(2)22FcH7 and the P element insertion KG03815
in CG3210 (Bellen et. al., 2004; Littleton and Bellen, 1994). CG3210 is
also known in Flybase as dynamin-2 (Lloyd et. al., 2000), noodle or drp1
(van der Bliek et. al., 1999). To further confirm that CG3210 corresponds
to frat, we sequenced the alleles and identified mutations in frat1, frat2
and l(2)22FcH7. Finally, we performed rescue experiments using a genomic
rescue construct that spans the CG3210 gene. This construct rescues the
lethality associated with homozygous frat2 and trans-heterozygous
frat1/frat2 flies. Hence, frat corresponds to CG3210 or drp1 and we
therefore renamed frat1 as drp11; frat2 as drp12; l(2)22FdT26 as
drp1T26;
l(2)22FcH7 as drp1H7 and KG03815 as drp1KG.
Hope this helps,
Cindy Ly
Bellen Lab
Department of Neuroscience
Baylor College of Medicine