FlyBase Allele Report: Dmel\Drp1[2]

General Information

Symbol

Dmel\Drp1²

Species

D. melanogaster

Name

FlyBase ID

FBal0176234

Feature type

allele

Associated gene

Dmel\Drp1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutation identified by sequence analysis.

(Ly, 2005.3.2)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone

(Smith et al., 2019)

abnormal neurophysiology

(Ly, 2005.3.2)

abnormal neurophysiology | adult stage

(Sandoval et al., 2014)

increased cell size | pupal stage (with Drp1¹)

(Anding et al., 2018)

lethal (with Drp1¹)

(Chao et al., 2016, Ly, 2005.3.2)

lethal | recessive

(Ly, 2005.3.2)

Phenotype Manifest In

axon | adult stage | somatic clone

(Smith et al., 2019)

cell body | adult stage | somatic clone

(Smith et al., 2019)

embryonic/larval neuromuscular junction

(Verstreken et al., 2005)

midgut | pupal stage (with Drp1¹)

(Anding et al., 2018)

mitochondrion

(Sandoval et al., 2014)

mitochondrion (with Drp1¹)

(Rival et al., 2011, Rival et al., 2011)

mitochondrion & bouton

(Verstreken et al., 2005)

mitochondrion & bouton (with Drp1¹)

(Verstreken et al., 2005)

mitochondrion & larval nervous system

(Verstreken et al., 2005)

mitochondrion & larval salivary gland

(Verstreken et al., 2005)

mitochondrion & motor neuron

(Verstreken et al., 2005)

mitochondrion | adult stage | somatic clone

(Smith et al., 2019)

mitochondrion | pupal stage (with Drp1¹)

(Anding et al., 2018)

Detailed Description

Statement

Reference

Drp1² homozygous mutant MARCM clones of glutamatergic neurons in the proximal wing have significantly increased mitochondrial length in the axons and mitochondrial hyperfusion in the cell bodies, compared to controls.

(Smith et al., 2019)

Drp1¹/Drp1² transheterozygote mutants are lethal.

(Chao et al., 2016)

Drp1² mutant retinal clones do not exhibit lipid droplet accumulation. Aconitase activity is reduced in these mutants and there is a strong correlation between decreased aconitase enzymatic activity and the number of lipid droplets per ommatidium, suggesting reactive oxygen species may play a role in lipid droplet accumulation.

(Liu et al., 2015)

The electroretinogram has a normal amplitude but shows loss of the on- and off-transients in mutant adults.

(Sandoval et al., 2014)

Drp1¹/Drp1² flies do not show clumping of mitochondria or muscle cell death in the indirect flight muscles.

(Yun et al., 2014)

In Drp1¹/Drp1² mutant adult flight muscles, the mitochondria are enlarged (being round in shape) and fewer in number compared to wild type but contain intact internal structures.

(Rival et al., 2011)

Drp1¹/Drp1² cells contain fewer mitochondria per cell than wild type and mitochondrial morphology is abnormal.

(Rival et al., 2011)

Stimulus-dependent or resting cytosolic Ca[2+] levels are not affected by the chronic reduction of mitochondria in motor neuron-terminals of Drp1² mutants.

(Chouhan et al., 2010)

Shows defects in neurotransmitter release.

(Ly, 2005.3.2)

Homozygous third larval instar salivary gland cells show clustering of mitochondria.

Homozygous third larval instar brains show reduced levels of mitochondria in the neuropil and clumps of mitochondria in the motor neuron cell bodies bordering the ventral nerve cord.

Fewer mitochondria than normal are seen in the axons of homozygous motor neurons and they form long threads that are rarely seen in controls.

Mitochondrial number per bouton is reduced at homozygous and Drp1¹/Drp1² neuromuscular junctions.

Bouton number per muscle are and synapse length per muscle area are not different from those of controls in Drp1² neuromuscular junctions, although neuromuscular junction branching is slightly increased.

Intracellular resting Ca[2+] in Drp1² boutons is approximately 2-fold higher than the level in controls.

Neurotransmission at the neuromuscular junction (measured by the excitatory junctional potential (EJP) amplitude) is not affected when stimulated at 1Hz in 0.6mM, 1mM or 5mM extracellular Ca[2+] at 22[o]C, or in 5mM Ca[2+] at 36[o]C, and is only elevated in 0.25mM extracellular Ca[2+] in Drp1² mutants. At 0.25mM extracellular Ca[2+], controls fail to evoke EJPs in 20% of the stimulations, whereas Drp1² animals only fail 4% of the time.

Drp1² mutants cannot maintain normal neurotransmission (measured by the EJP) during high frequency (10Hz) stimulation at 22[o]C in contrast to controls. This phenotype is exacerbated at 36[o]C and can be partially rescued by ATP.

Endocytosis and exocytosis of endo cycling pool vesicles at the neuromuscular junction is not disrupted in stimulated Drp1² mutants, but the mutants show a defect in the cycling of reserve pool vesicles.

(Verstreken et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Drp1²/Drp1¹ has lethal phenotype, suppressible by Scer\GAL4^da.PU/Hsap\DNM1L^UAS.cCa

(Chao et al., 2016)

Drp1²/Drp1¹ has lethal phenotype, suppressible by Scer\GAL4^da.PU/Hsap\DNM1L^E379K.UAS

(Chao et al., 2016)

NOT suppressed by

Statement

Reference

Drp1²/Drp1¹ has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Hsap\DNM1L^A395D.UAS

(Chao et al., 2016)

Drp1²/Drp1¹ has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Hsap\DNM1L^G350R.UAS

(Chao et al., 2016)

NOT Suppressor of

Statement

Reference

Drp1²/Drp1[+] is a non-suppressor of visible phenotype of Pink1^B9

(Park et al., 2009)

Drp1²/Drp1[+] is a non-suppressor of flightless phenotype of Pink1^B9

(Park et al., 2009)

Drp1²/Drp1[+] is a non-suppressor of flightless phenotype of park¹

(Park et al., 2009)

Drp1²/Drp1[+] is a non-suppressor of visible phenotype of park¹

(Park et al., 2009)

Other

Statement

Reference

Df(3L)Pmi-PGRP-LD, Drp1²/Drp1¹ has lethal phenotype

(Rival et al., 2011, Rival et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Drp1²/Drp1¹ has mitochondrion phenotype, suppressible by Scer\GAL4^elav.PU/Marf^RNAi.CDS.UAS

(Rival et al., 2011)

Drp1²/Drp1¹ has mitochondrion phenotype, suppressible by Marf^RNAi.UTR.UAS/Scer\GAL4^elav.PU

(Rival et al., 2011)

Drp1²/Drp1¹ has mitochondrion phenotype, suppressible by Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS

(Rival et al., 2011)

NOT suppressed by

Statement

Reference

Drp1²/Drp1¹ has mitochondrion phenotype, non-suppressible by Scer\GAL4^elav.PU/Pmi^UAS.cRa

(Rival et al., 2011)

Suppressor of

Statement

Reference

Drp1² is a suppressor of neuroblast | increased number | somatic clone phenotype of N^CA.UAS.cUa, Scer\GAL4^Act5C.PU

(Lee et al., 2013)

NOT Suppressor of

Statement

Reference

Drp1²/Drp1¹ is a non-suppressor of mitochondrion | third instar larval stage phenotype of Scer\GAL4^how-24B, mask^HMS01045

(Zhu et al., 2015)

Drp1²/Drp1¹ is a non-suppressor of larval somatic muscle cell | third instar larval stage phenotype of Scer\GAL4^how-24B, mask^HMS01045

(Zhu et al., 2015)

Drp1²/Drp1[+] is a non-suppressor of wing phenotype of park¹

(Park et al., 2009)

Drp1²/Drp1[+] is a non-suppressor of adult thorax phenotype of park¹

(Park et al., 2009)

Drp1²/Drp1[+] is a non-suppressor of wing phenotype of Pink1^B9

(Park et al., 2009)

Drp1²/Drp1[+] is a non-suppressor of adult thorax phenotype of Pink1^B9

(Park et al., 2009)

Drp1²/Drp1[+] is a non-suppressor of mitochondrion & dopaminergic neuron phenotype of Pink1^B9

(Park et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced connectivity of the mitochondrial network in body wall muscles characteristic for third instar larvae expressing mask^HMS01045 under the control of Scer\GAL4^how-24B cannot is not changed by combination with Drp1¹/Drp1².

(Zhu et al., 2015)

Drp1² suppresses the increase in the number of neuroblasts per clone seen in clones expressing N^{int.U.Scer\UAS} under the control of Scer\GAL4^Act5C.PU.

(Lee et al., 2013)

Expression of either Marf^{miRNA.CDS.Scer\UAS}, Marf^{miRNA.UTR.Scer\UAS} or opa1-like^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^elav.PU is sufficient to restore a normal filamentous mitochondrial network in Drp1¹/Drp1² flies.

Expression of Pmi^Scer\UAS.cRa under the control of Scer\GAL4^elav.PU does not rescue mitochondrial morphology in Drp1¹/Drp1² flies.

(Rival et al., 2011)

Flies expressing porin^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Mef2.PR in a Drp1²/+ background show defects in mitochondrial morphology in the indirect flight muscles, with smaller or almost completely abolished mitochondria.

(Park et al., 2010)

The downturned wing, crushed thorax and flightless phenotypes of Pink1^B9 mutant flies are not suppressed by Drp1²/+.

The swollen mitochondria phenotype seen in dopaminergic neurons of Pink1^B9 mutants is not suppressed by Drp1²/+.

(Park et al., 2009)

Xenogenetic Interactions

Statement

Reference

The lethality of Drp1¹/Drp1² transheterozygotes is rescued by expression of Hsap\DNM1L^Scer\UAS.cCa under the control of Scer\GAL4^da.PU in the mutant background.

The lethality of Drp1¹/Drp1² transheterozygotes is not rescued by expression of Hsap\DNM1L^{A395D.Scer\UAS} under the control of Scer\GAL4^da.PU in the mutant background.

The lethality of Drp1¹/Drp1² transheterozygotes is not rescued by expression of Hsap\DNM1L^{G350R.Scer\UAS} under the control of Scer\GAL4^da.PU in the mutant background.

The lethality of Drp1¹/Drp1² transheterozygotes is rescued by expression of Hsap\DNM1L^{E379K.Scer\UAS} under the control of Scer\GAL4^da.PU in the mutant background.

(Chao et al., 2016)

Complementation and Rescue Data

Fails to complement

(Ly, 2005.3.2)

(Ly, 2005.3.2)

(Ly, 2005.3.2)

(Ly, 2005.3.2)

(Verstreken et al., 2005)

Rescued by

Drp1²/Drp1¹ is rescued by Drp1^UAS.cUa

(Chao et al., 2016)

Drp1²/Drp1¹ is rescued by Drp1^UAS.cDa/Scer\GAL4^elav.PU

(Rival et al., 2011)

Drp1² is rescued by Drp1^{+tCH322-83H15}

(Venken et al., 2009)

Drp1²/Drp1¹ is rescued by Drp1^{Tag:FLAG,Tag:CALI(TC),Tag:HA}

(Verstreken et al., 2005)

Drp1²/Drp1¹ is rescued by Drp1^+t9.4

(Verstreken et al., 2005)

Drp1² is rescued by Drp1^{Tag:FLAG,Tag:CALI(TC),Tag:HA}

(Verstreken et al., 2005)

Drp1² is rescued by Drp1^+t9.4

(Verstreken et al., 2005)

Comments

The lethality of Drp1¹/Drp1² transheterozygotes is rescued by expression of Drp1^Scer\UAS.cUa under the control of Scer\GAL4^da.PU in the mutant background.

(Chao et al., 2016)

Expression of Drp1^Scer\UAS.cDa under the control of Scer\GAL4^elav.PU rescues mitochondrial morphology in Drp1¹/Drp1² flies.

(Rival et al., 2011)

Lethality of Drp1² and Drp1¹/Drp1² rescued by a genomic rescue construct spanning the 'CG3210' annotation.

(Ly, 2005.3.2)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24899

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{Car20y}25F Drp1² P{neoFRT}40A/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

(Vos et al., 2013)

Drp1²

(Smith et al., 2019, Anding et al., 2018, Chao et al., 2016, Liu et al., 2015, Zhu et al., 2015, Sandoval et al., 2014, Lee et al., 2013, Imai et al., 2010, Venken et al., 2009)

drp1²

(Insolera et al., 2021, Assia Batzir et al., 2019, Yun et al., 2014, Rival et al., 2011, Rival et al., 2011, Chouhan et al., 2010, Park et al., 2010, Park et al., 2009, Deng et al., 2008, Yang et al., 2008, Ly, 2005.3.2, Verstreken et al., 2005)

frat²

(Ly, 2005.3.2, Verstreken et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (22)

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