FlyBase Allele Report: Dmel\Drp1[1]

General Information

Symbol

Dmel\Drp1¹

Species

D. melanogaster

Name

FlyBase ID

FBal0176235

Feature type

allele

Associated gene

Dmel\Drp1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutation identified by sequence analysis.

(Ly, 2005.3.2)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of encephalopathy due to defective mitochondrial and peroxisomal fission 1

CEA with Hsap\DNM1L^A395D.UAS

(Chao et al., 2016)

CEA with Hsap\DNM1L^G350R.UAS

(Chao et al., 2016)

CEA with Hsap\DNM1L^Y691C.UAS

(Assia Batzir et al., 2019)

DOES NOT model encephalopathy due to defective mitochondrial and peroxisomal fission 1

CEA with Hsap\DNM1L^E379K.UAS

(Chao et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{G298S.UAS.Tag:FLAG}

(Khalil et al., 2017)

modeled by Hsap\TARDBP^{UAS.cKa.Tag:FLAG}

(Khalil et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone

(Smith et al., 2019)

abnormal neurophysiology

(Ly, 2005.3.2)

increased cell size | pupal stage (with Drp1²)

(Anding et al., 2018)

lethal (with Df(2L)ED134)

(Kahsai and Cook, 2018)

lethal (with Df(2L)Exel6008)

(Kahsai and Cook, 2018)

lethal (with Drp1²)

(Chao et al., 2016, Ly, 2005.3.2)

Phenotype Manifest In

axon | adult stage | somatic clone

(Smith et al., 2019)

cell body | adult stage | somatic clone

(Smith et al., 2019)

midgut | pupal stage (with Drp1²)

(Anding et al., 2018)

mitochondrion (with Drp1²)

(Rival et al., 2011, Rival et al., 2011)

mitochondrion & bouton (with Drp1²)

(Verstreken et al., 2005)

mitochondrion & larval salivary gland

(Verstreken et al., 2005)

mitochondrion | adult stage | somatic clone

(Smith et al., 2019)

mitochondrion | pupal stage (with Drp1²)

(Anding et al., 2018)

Detailed Description

Statement

Reference

Drp1¹ homozygous mutant MARCM clones of glutamatergic neurons in the proximal wing have significantly increased mitochondrial length in the axons and mitochondrial hyperfusion in the cell bodies, compared to controls.

(Smith et al., 2019)

Drp1¹/Drp1² transheterozygote mutants are lethal.

(Chao et al., 2016)

Drp1¹/Drp1² flies do not show clumping of mitochondria or muscle cell death in the indirect flight muscles.

(Yun et al., 2014)

In Drp1¹/Drp1² mutant adult flight muscles, the mitochondria are enlarged (being round in shape) and fewer in number compared to wild type but contain intact internal structures.

(Rival et al., 2011)

Drp1¹/Drp1² cells contain fewer mitochondria per cell than wild type and mitochondrial morphology is abnormal.

(Rival et al., 2011)

Shows defects in neurotransmitter release.

(Ly, 2005.3.2)

Homozygous third instar salivary gland cells show clustering of mitochondria.

Mitochondrial number per bouton is reduced at Drp1¹/Drp1² neuromuscular junctions.

(Verstreken et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Drp1²/Drp1¹ has lethal phenotype, suppressible by Scer\GAL4^da.PU/Hsap\DNM1L^UAS.cCa

(Chao et al., 2016)

Drp1²/Drp1¹ has lethal phenotype, suppressible by Scer\GAL4^da.PU/Hsap\DNM1L^E379K.UAS

(Chao et al., 2016)

NOT suppressed by

Statement

Reference

Drp1²/Drp1¹ has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Hsap\DNM1L^A395D.UAS

(Chao et al., 2016)

Drp1²/Drp1¹ has lethal phenotype, non-suppressible by Scer\GAL4^da.PU/Hsap\DNM1L^G350R.UAS

(Chao et al., 2016)

Other

Statement

Reference

Drp1¹, Hsap\DNM1L^A395D.UAS, Scer\GAL4^Mef2.PU has lethal phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^G350R.UAS, Scer\GAL4^Toll-6-D42 has abnormal neuroanatomy | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^E379K.UAS, Scer\GAL4^Toll-6-D42 has abnormal neuroanatomy | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^A395D.UAS, Scer\GAL4^Act5C.PI has lethal phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^G350R.UAS, Scer\GAL4^Act5C.PI has lethal phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^A395D.UAS, Scer\GAL4^Toll-6-D42 has abnormal neuroanatomy | third instar larval stage phenotype

(Chao et al., 2016)

Df(3L)Pmi-PGRP-LD, Drp1²/Drp1¹ has lethal phenotype

(Rival et al., 2011, Rival et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Drp1²/Drp1¹ has mitochondrion phenotype, suppressible by Scer\GAL4^elav.PU/Marf^RNAi.CDS.UAS

(Rival et al., 2011)

Drp1²/Drp1¹ has mitochondrion phenotype, suppressible by Marf^RNAi.UTR.UAS/Scer\GAL4^elav.PU

(Rival et al., 2011)

Drp1²/Drp1¹ has mitochondrion phenotype, suppressible by Scer\GAL4^elav.PU/Opa1^RNAi.CDS.UAS

(Rival et al., 2011)

NOT suppressed by

Statement

Reference

Drp1²/Drp1¹ has mitochondrion phenotype, non-suppressible by Scer\GAL4^elav.PU/Pmi^UAS.cRa

(Rival et al., 2011)

NOT Suppressor of

Statement

Reference

Drp1²/Drp1¹ is a non-suppressor of mitochondrion | third instar larval stage phenotype of Scer\GAL4^how-24B, mask^HMS01045

(Zhu et al., 2015)

Drp1²/Drp1¹ is a non-suppressor of larval somatic muscle cell | third instar larval stage phenotype of Scer\GAL4^how-24B, mask^HMS01045

(Zhu et al., 2015)

Other

Statement

Reference

Drp1¹, Hsap\DNM1L^A395D.UAS, Scer\GAL4^Mef2.PU has larval somatic muscle cell | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^G350R.UAS, Scer\GAL4^Toll-6-D42 has bouton | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^G350R.UAS, Scer\GAL4^Toll-6-D42 has axon | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^G350R.UAS, Scer\GAL4^Toll-6-D42 has larval ventral nerve cord | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^G350R.UAS, Scer\GAL4^Toll-6-D42 has mitochondrion | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^E379K.UAS, Scer\GAL4^Toll-6-D42 has bouton | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^E379K.UAS, Scer\GAL4^Toll-6-D42 has mitochondrion | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^A395D.UAS, Scer\GAL4^Mef2.PU has mitochondrion | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^G350R.UAS, Scer\GAL4^Mef2.PU has larval somatic muscle cell | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^G350R.UAS, Scer\GAL4^Mef2.PU has mitochondrion | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^A395D.UAS, Scer\GAL4^Toll-6-D42 has bouton | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^A395D.UAS, Scer\GAL4^Toll-6-D42 has axon | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^A395D.UAS, Scer\GAL4^Toll-6-D42 has larval ventral nerve cord | third instar larval stage phenotype

(Chao et al., 2016)

Drp1¹, Hsap\DNM1L^A395D.UAS, Scer\GAL4^Toll-6-D42 has mitochondrion | third instar larval stage phenotype

(Chao et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced connectivity of the mitochondrial network in body wall muscles characteristic for third instar larvae expressing mask^HMS01045 under the control of Scer\GAL4^how-24B cannot is not changed by combination with Drp1¹/Drp1².

(Zhu et al., 2015)

Expression of either Marf^{miRNA.CDS.Scer\UAS}, Marf^{miRNA.UTR.Scer\UAS} or opa1-like^{miRNA.CDS.Scer\UAS} under the control of Scer\GAL4^elav.PU is sufficient to restore a normal filamentous mitochondrial network in Drp1¹/Drp1² flies.

Expression of Pmi^Scer\UAS.cRa under the control of Scer\GAL4^elav.PU does not rescue mitochondrial morphology in Drp1¹/Drp1² flies.

(Rival et al., 2011)

Xenogenetic Interactions

Statement

Reference

The lethality of Drp1¹/Drp1² transheterozygotes is rescued by expression of either Hsap\DNM1L^Scer\UAS.cCa or Hsap\DNM1L^{E379K.Scer\UAS} under the control of Scer\GAL4^da.PU in the mutant background.

The lethality of Drp1¹/Drp1² transheterozygotes is not rescued by expression of either Hsap\DNM1L^{A395D.Scer\UAS} or Hsap\DNM1L^{G350R.Scer\UAS} under the control of Scer\GAL4^da.PU in the mutant background.

Expression of either Hsap\DNM1L^Scer\UAS.cCa or Hsap\DNM1L^{E379K.Scer\UAS} under the control of either Scer\GAL4^Act5C.PI or Scer\GAL4^Mef2.PU does not cause lethality when expressed in sensitised Drp1¹/+ background.

Expression of Hsap\DNM1L^{A395D.Scer\UAS} under the control of either Scer\GAL4^Act5C.PI or Scer\GAL4^Mef2.PU causes lethality when expressed in sensitised Drp1¹/+ background. Expression of Hsap\DNM1L^{G350R.Scer\UAS} causes lethality only when driven by Scer\GAL4^Act5C.PI but not when driven by Scer\GAL4^Mef2.PU.

Expression of Hsap\DNM1L^Scer\UAS.cCa under the control of Scer\GAL4^Mef2.PU does not cause any defects in the morphology or number of mitochondria in third instar larval muscles when expressed in sensitised Drp1¹/+ background. Expression under the control of Scer\GAL4^Toll-6-D42 also does not cause any mitochondria trafficking defects in the ventral nerve cord, axons and synaptic boutons (though number of mitochondria per bouton is slightly lower compared to controls) in third instar larvae.

Expression of Hsap\DNM1L^{A395D.Scer\UAS} under the control of Scer\GAL4^Mef2.PU in sensitised Drp1¹/+ background causes mitochondria morphological defects (large blocks of interconnected mitochondria) as well as defects in their distribution, leading to decreased number of mitochondria per sarcomere or per muscle area in third instar larval muscles. Expression under the control of Scer\GAL4^Toll-6-D42 leads to mitochondria trafficking defects in the ventral nerve cord, axons and synaptic boutons in third instar larvae. The number of mitochondria per axon area or per bouton is significantly decreased compared to controls.

Expression of Hsap\DNM1L^{G350R.Scer\UAS} under the control of Scer\GAL4^Mef2.PU in sensitised Drp1¹/+ background causes mitochondria morphological defects (large blocks of interconnected mitochondria) as well as defects in their distribution, leading to decreased number of mitochondria per sarcomere or per muscle area in third instar larval muscles. Expression under the control of Scer\GAL4^Toll-6-D42 also leads to mitochondria trafficking defects in the ventral nerve cord, axons and synaptic boutons in third instar larvae. The number of mitochondria per axon area or per bouton is significantly decreased compared to controls.

Expression of Hsap\DNM1L^{E379K.Scer\UAS} under the control of Scer\GAL4^Mef2.PU in sensitised Drp1¹/+ background does not cause any defects in the morphology or number of mitochondria in third instar larval muscles. Expression under the control of Scer\GAL4^Toll-6-D42 also does not cause mitochondria trafficking defects in the ventral nerve cord and axons but leads to clear trafficking defect at the level of synaptic boutons (number of mitochondria per bouton is significantly decreased compared to controls) in third instar larvae.

(Chao et al., 2016)

Complementation and Rescue Data

Fails to complement

(Ly, 2005.3.2)

(Ly, 2005.3.2)

(Ly, 2005.3.2)

(Ly, 2005.3.2)

(Verstreken et al., 2005)

Rescued by

Drp1²/Drp1¹ is rescued by Drp1^UAS.cUa

(Chao et al., 2016)

Drp1²/Drp1¹ is rescued by Drp1^UAS.cDa/Scer\GAL4^elav.PU

(Rival et al., 2011)

Drp1²/Drp1¹ is rescued by Drp1^{Tag:FLAG,Tag:CALI(TC),Tag:HA}

(Verstreken et al., 2005)

Drp1²/Drp1¹ is rescued by Drp1^+t9.4

(Verstreken et al., 2005)

Comments

The lethality of Drp1¹/Drp1² transheterozygotes is rescued by expression of Drp1^Scer\UAS.cUa under the control of Scer\GAL4^da.PU in the mutant background.

(Chao et al., 2016)

Expression of Drp1^Scer\UAS.cDa under the control of Scer\GAL4^elav.PU rescues mitochondrial morphology in Drp1¹/Drp1² flies.

(Rival et al., 2011)

Lethality of Drp1¹/Drp1² rescued by a genomic rescue construct spanning the 'CG3210' annotation.

(Ly, 2005.3.2)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24885

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{Car20y}25F Drp1¹ P{neoFRT}40A/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DRP1¹

(Khalil et al., 2017)

Drp1¹

(Cao et al., 2021, Smith et al., 2019, Anding et al., 2018, Kahsai and Cook, 2018, Chao et al., 2016, Zhu et al., 2015)

drp1¹

(Insolera et al., 2021, Lin et al., 2021, Assia Batzir et al., 2019, Yun et al., 2014, Vos et al., 2013, Rival et al., 2011, Rival et al., 2011, Deng et al., 2008, Ly, 2005.3.2, Verstreken et al., 2005)

frat¹

(Ly, 2005.3.2, Verstreken et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (17)

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