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Citation
Stultz, B.G., Jackson, D.G., Mortin, M.A., Yang, X., Beachy, P.A., Hursh, D.A. (2006). Transcriptional activation by extradenticle in the Drosophila visceral mesoderm.  Dev. Biol. 290(2): 482--494.
FlyBase ID
FBrf0190166
Publication Type
Research paper
Abstract

decapentaplegic (dpp) is a direct target of Ultrabithorax (Ubx) in parasegment 7 (PS7) of the embryonic visceral mesoderm. We demonstrate that extradenticle (exd) and homothorax (hth) are also required for dpp expression in this location, as well as in PS3, at the site of the developing gastric caecae. A 420 bp element from dpp contains EXD binding sites necessary for expressing a reporter gene in both these locations. Using a specificity swap, we demonstrate that EXD directly activates this element in vivo. Activation does not require Ubx, demonstrating that EXD can activate transcription independently of homeotic proteins. Restoration is restricted to the domains of endogenous dpp expression, despite ubiquitous expression of altered specificity EXD. We demonstrate that nuclear EXD is more extensively phosphorylated than the cytoplasmic form, suggesting that EXD is a target of signal transduction by protein kinases.

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PubMed Central ID
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Secondary IDs
  • FBrf0193896
Language of Publication
English
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Parent Publication
Publication Type
Journal
Abbreviation
Dev. Biol.
Title
Developmental Biology
Publication Year
1959-
ISBN/ISSN
0012-1606
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