Subject: question about vasa mutations Dear Dr. Lasko, I am a curator for FlyBase and am working on the annotation of the genomic sequence. I am currently working on vasa and wanted to include the sites of the mutations from the Liang et. al. 1994 Development paper. I can't seem to find amino acid coordinates for some of the mutations mapped in the paper. I have found the following changes vasHE1 (vasHE in paper) R170S vas4 (vasO11</up> in paper) I271N vasAS H520Y vas5 (vasO14 in paper) I257M (the amino acid change is said to be in codon 256 but the nucleotide change is supposed to be ATA -> ATG. I255 and I256 are ATT and I257 is ATA so I guessed that the change is in I257. Can you confirm?) Can you tell me what codons are changed in vas3F, vas4C, vasD5, vasPW72 (vasPW in paper), and vas6 (vasQ6 in paper)? Also can you tell me exactly where the 4bp insertion is in vasQS17? Any information you can provide will be kept as a personal communication to FlyBase and used as a reference for the annotation. Thanks for your help. Sincerely, Beverley Matthews Subject: Re: question about vasa mutations Dear Beverley, Here are the correct amino acid coordinates for the vas mutations: vasHE1 R170S vasAS H520Y vas3F G587E vas4C P268S vasD5 G552E vasPW72 S518F vasQ6 V465M vasO14 I271M vasO11 I256N I'll be back about the QS17 mutation, that will take some digging... All the best, Paul.
