Isolation and characterization of Df(2R)BSC265
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC265 was isolated as a FLP recombinase-induced recombination event involving P{XP}d10323 and PBac{WH}CG8728f01392. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d10323/PBac{WH}CG8728f01392 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC265 from the segment of P{XP}d10323 to the left of its FRT site and the segment of PBac{WH}CG8728f01392 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d10323 to be at Release 3 genomic coordinate 2843728 on chromosome arm 2R. This corresponds to 43E16 on the Release 3 and 4 genome maps. The predicted position of PBac{WH}CG8728f01392on the Release 4 map is 43F4 . Consequently, the cytological breakpoints of Df(2R)BSC265 are predicted to be 43E16;43F4.  It failed to complement sax5, lin19k01207 and rnh1k07624.