Isolation and characterization of Df(2R)BSC298
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC298 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG1776f00802 and P{XP}Mef2d07371. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG1776f00802/P{XP}Mef2d07371 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC298 from the segment of PBac{WH}CG1776f00802  to the left of its FRT site and the segment of P{XP}Mef2d07371 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC298 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 46B2;46C7. It failed to complement Mef2X1, Df(2R)BSC131 and Df(2R)BSC133.