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Christensen, S., Cook, K. (2007.3.22). Isolation and characterization of Df(2L)BSC254. 
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FBrf0199294
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Date: Thu, 22 Mar 2007  14:52:09  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2L)BSC254
Isolation and characterization of Df(2L)BSC254
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC254 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f04398 and P{XP}d09327. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}f04398/P{XP}d09327 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC254 from the segment of 
PBac{WH}f04398 to the left of its FRT site and the segment of 
P{XP}d09327 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. The 
cytological breakpoints of Df(2L)BSC254 predicted from the Release 4 
genomic coordinates of the transposable element insertions sites are 
35B6;35C1. It failed to complement l(2)35Bb[k11524a], 
l(2)35Bd[10408], ck[13] and vas[1].
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    English
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    Aberrations (1)
    Alleles (4)
    Genes (4)
    Insertions (3)
    Transgenic Constructs (1)