Isolation and characterization of Df(2R)BSC356
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC356 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}ykif06249 and P{XP}CG3376d09724. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}ykif06249/P{XP}CG3376d09724 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC356 from the segment of PBac{WH}ykif06249 to the left of its FRT site and the segment of P{XP}CG3376d09724 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC356 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 60B8;60C4. It failed to complement gek09373 and spagk12101.