FlyBase Allele Report: Dmel\spag[k12101]

General Information

Symbol

Dmel\spag^k12101

Species

D. melanogaster

Name

FlyBase ID

FBal0087051

Feature type

allele

Associated gene

Dmel\spag

Associated Insertion(s)

P{lacW}spag^k12101

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Roch et al., 1998, Iliopoulos et al., 1997, BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}spag^k12101

Cytology

Description

P{lacW}spag^k12101 is inserted 52 nucleotides downstream from the spag ORF initiation codon.

(Benbahouche et al., 2014)

P{lacW} insertion 52 nucleotides downstream from the translation initiation codon of the presumed open reading frame and 382 nucleotides upstream from the 5' end of DnaJ-60 cDNA.

(Iliopoulos et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval fat body

(Hauling et al., 2014)

embryonic/larval lymph gland

(Benbahouche et al., 2014, Minakhina and Steward, 2006, Iliopoulos et al., 1997)

embryonic/larval lymph gland (with In(2LR)Px⁴)

(Benbahouche et al., 2014)

embryonic/larval lymph gland (with spag^ΔR7)

(Benbahouche et al., 2014)

imaginal disc

(Minakhina and Steward, 2006, Roch et al., 1998, Iliopoulos et al., 1997)

lamellocyte

(Hauling et al., 2014)

melanotic mass

(Minakhina and Steward, 2006)

melanotic mass & hemocoel

(Iliopoulos et al., 1997)

secondary lobe of embryonic/larval lymph gland

(Benbahouche et al., 2014)

secondary lobe of embryonic/larval lymph gland (with In(2LR)Px⁴)

(Benbahouche et al., 2014)

secondary lobe of embryonic/larval lymph gland (with spag^ΔR7)

(Benbahouche et al., 2014)

tertiary lobe of embryonic/larval lymph gland

(Benbahouche et al., 2014)

tertiary lobe of embryonic/larval lymph gland (with In(2LR)Px⁴)

(Benbahouche et al., 2014)

tertiary lobe of embryonic/larval lymph gland (with spag^ΔR7)

(Benbahouche et al., 2014)

trichome

(Roch et al., 1998)

wing

(Roch et al., 1998)

wing disc | larval stage | somatic clone

(Roch et al., 1998)

Detailed Description

Statement

Reference

Homozygous spag^k12101 larvae die 6-7 days after egg laying, with overgrowth of the hematopoietic organs and occasional formation of melanotic pseudo-tumours. This occurs more often in the distal and secondary lobes, which are usually the smallest in wild-type larvae. All other tissue such as imaginal discs and salivary glands are reduced in size.

(Benbahouche et al., 2014)

spag^k12101 mutant fat bodies exhibit an increase in lamellocytes.

(Hauling et al., 2014)

Mutant third instar larvae have multiple melanotic nodules surrounded by hemocytes. The nodules are located symmetrically along the body wall and often replace imaginal discs. The melanotic nodules are surrounded by lamellocytes. The lymph glands are about twice the size of normal lymph glands in the mutant larvae.

(Minakhina and Steward, 2006)

Mutation causes abnormal trichome differentiation and small cells. Mutants exhibit visible disc abnormalities: very small or no discs. Clones induced in wild type discs are abnormal: clones are generated at a lower than expected frequency and are small with a significantly elongated shape along the proximodistal axis. Clones in the wing cause non-autonomous effects on the proliferation of neighbouring territories, leading eventually to a reduction in wing size.

(Roch et al., 1998)

Homozygotes die at the larval to pupal transition phase with overgrowth of the haematopoietic organs, formation of secondary melanotic tumours in the haemocoel and atrophy of the imaginal discs.

(Iliopoulos et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

spag^k12101/spag^ΔR7 has melanotic mass phenotype phenotype, non-suppressible by DnaJ-60^t4.3

(Benbahouche et al., 2014)

spag^k12101/In(2LR)Px⁴ has melanotic mass phenotype phenotype, non-suppressible by DnaJ-60^t4.3