Isolation and characterization of Df(1)BSC542
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC542 was isolated as a FLP recombinase-induced recombination event involving P{XP}d09482 and PBac{WH}f06054. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{XP}d09482/PBac{WH}f06054; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC542 from the segment of P{XP}d09482 to the left of its FRT site and the segment of PBac{WH}f06054 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d09482 to be Release 3 genomic coordinate 11651944 on the X chromosome. This corresponds to 10F7 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}f06054 on the Release 5 map is 11A3. Consequently, the cytological breakpoints of Df(1)BSC542 are predicted to be 10F7;11A3. It failed to complement gd7 and fw1.