Df(3R)BSC789 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}e00251 and P{XP}Pkc98Ed08513. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{RB}e00251/P{XP}Pkc98Ed08513 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC789 from the segment of PBac{RB}e00251 to the left of its FRT site and the segment of P{XP}Pkc98Ed08513 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(3R)BSC789 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:24645856 ;24866229 and the cytological breakpoints predicted from these coordinates are 98E5;98F6. Df(3R)BSC789 failed to complement ApcQ8 and Doa3.
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University