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General Information
Symbol
Dmel\Apc2N175K
Species
D. melanogaster
Name
FlyBase ID
FBal0148480
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

T24164698R

Amino acid change:

N175K | Apc2-PA; N155K | Apc2-PB; N175K | Apc2-PC

Reported amino acid change:

N175K

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: N175K.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

12% of Apc2N175K maternally mutant syncytial embryos show movement of over 2% of cortical nuclei into the embryo interior, compared to 0-3% of wild-type embryos.

The cuticle of an average Apc2N175K maternal/zygotic embryo shows an anterior hole, is 50-60% the length of wild-type and has 3 patches of denticles remaining.

Homozygous flies have wing vein and bristle defects and slightly rough eyes. Mutant females lay very few eggs and only some of these eggs develop through late embryonic stages. These escaper embryos produce cuticles that are completely naked on the ventral side. Ovaries of homozygous females contain very few mature egg chambers and oocytes are often underdeveloped in large chambers. The nurse cells tend to be irregularly arranged within maturing egg chambers. In 17% of egg chambers, the oocyte is mispositioned to one side of the anterior-posterior axis, rather than occupying the most posterior position of the egg chamber as in wild type. Mutant egg chambers have fewer membrane stacks in the plasma membranes of the germ cells than wild type, or abnormal stacks, showing long stretches of thin straight membrane interfaces that are devoid of membrane interdigitations. Cell vertices and ring canals also contain fewer membrane stacks than normal or abnormal membrane stacks, tending to have a flimsy appearance. There are numerous gaps between nurse cell membranes, especially at three-cell joins, the most abnormal of which have gaping holes. The number of spot adherens junctions is reduced to 31% of wild type in the epithelial cells of stage 6/7 embryos derived from Apc2N175K parents. These embryos have extensive gaps between the lateral plasma membranes and large gaping holes at three-cell joins.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Apc2N175K, ApcQ8 double homozygous mutant clones (induced using Scer\FLP1SOP.scE1x6) have a failure to prune all ddaC dendrites by 16 h after puparium formation (APF), unlike controls.

Clonal Apc2N175K, ApcQ8 double homozygosity alone or clonal Apc2N175K, ApcQ8 double homozygosity in combination with clonal SxlKK116156 co-expression do not enhance the increased mitotic index in the adult posterior midgut induced by the clonal expression of Ras85DV12.UAS under the control of Scer\GAL4esg-NP7397.

Embryos maternally and zygotically double mutant for ApcQ8 and Apc2N175K display a naked cuticle phenotype.

Expression of AxnScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS restores normal-looking denticle belts in embryos maternally and zygotically mutant for ApcQ8 and Apc2N175K.

ApcQ8, Apc2N175K double mutant embryos have a naked cuticle phenotype.

The penetrance of the oocyte mislocalisation phenotype see in Apc2N175K mutant females is increased to 32% if they are also mutant for ApcQ8.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Partially rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Apc2 alleles can be divided into three categories based on their embryonic cuticle phenotypes, from weak to moderate to strong: Apc2e90 = Apc2b5 = Apc2N175K < Apc2c9 = Apc2ΔS = Apc2d40 < Apc2g41 = Apc2f90 = Apc2g10.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (13)