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Citation
Hu, W., Wan, D., Yu, X., Cao, J., Guo, P., Li, H.S., Han, J. (2012). Protein gq modulates termination of phototransduction and prevents retinal degeneration.  J. Biol. Chem. 287(17): 13911--13918.
FlyBase ID
FBrf0218114
Publication Type
Research paper
Abstract

Appropriate termination of the phototransduction cascade is critical for photoreceptors to achieve high temporal resolution and to prevent excessive Ca(2+)-induced cell toxicity. Using a genetic screen to identify defective photoresponse mutants in Drosophila, we isolated and identified a novel Gα(q) mutant allele, which has defects in both activation and deactivation. We revealed that G(q) modulates the termination of the light response and that metarhodopsin/G(q) interaction affects subsequent arrestin-rhodopsin (Arr2-Rh1) binding, which mediates the deactivation of metarhodopsin. We further showed that the Gα(q) mutant undergoes light-dependent retinal degeneration, which is due to the slow accumulation of stable Arr2-Rh1 complexes. Our study revealed the roles of G(q) in mediating photoresponse termination and in preventing retinal degeneration. This pathway may represent a general rapid feedback regulation of G protein-coupled receptor signaling.

PubMed ID
PubMed Central ID
PMC3340195 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Biol. Chem.
    Title
    Journal of Biological Chemistry
    Publication Year
    1905-
    ISBN/ISSN
    0021-9258
    Data From Reference
    Aberrations (1)
    Alleles (8)
    Genes (7)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (3)