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Citation
Lee, H., Peng, Y., Guo, Y. (2013). Analysis of Lipid Droplet Dynamics and Functions in Drosophila melanogaster.  Methods Cell Biol. 116C(): 53--69.
FlyBase ID
FBrf0222954
Publication Type
Research paper
Abstract

The lipid droplet (LD) is a unique cellular organelle containing a neutral-lipid core enclosed by a phospholipid monolayer and associated proteins. Despite the important function of LDs at the hub of cellular energy homeostasis regulation, major questions in the field of LD biology are still unanswered. Drosophila melanogaster has been used as a model organism to make fundamental discoveries in biology for over a century. In recent years, genome-wide unbiased reverse genetic screens using Drosophila cells or transgenic lines have been proven to provide valuable knowledge to the field of LD biology. Here we summarize the methods we use for functional genomic screens in Drosophila S2 cells to identify genes involved in LD biology, and the methods used for studying LD function in vivo using Drosophila as a model to combat metabolic diseases.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Methods Cell Biol.
    Title
    Methods in Cell Biology
    Publication Year
    1973-
    ISBN/ISSN
    0091-679X
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