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Citation
Zhang, W., Cheng, L.E., Kittelmann, M., Li, J., Petkovic, M., Cheng, T., Jin, P., Guo, Z., Göpfert, M.C., Jan, L.Y., Jan, Y.N. (2015). Ankyrin Repeats Convey Force to Gate the NOMPC Mechanotransduction Channel.  Cell 162(6): 1391--1403.
FlyBase ID
FBrf0229550
Publication Type
Research paper
Abstract

How metazoan mechanotransduction channels sense mechanical stimuli is not well understood. The NOMPC channel in the transient receptor potential (TRP) family, a mechanotransduction channel for Drosophila touch sensation and hearing, contains 29 Ankyrin repeats (ARs) that associate with microtubules. These ARs have been postulated to act as a tether that conveys force to the channel. Here, we report that these N-terminal ARs form a cytoplasmic domain essential for NOMPC mechanogating in vitro, mechanosensitivity of touch receptor neurons in vivo, and touch-induced behaviors of Drosophila larvae. Duplicating the ARs elongates the filaments that tether NOMPC to microtubules in mechanosensory neurons. Moreover, microtubule association is required for NOMPC mechanogating. Importantly, transferring the NOMPC ARs to mechanoinsensitive voltage-gated potassium channels confers mechanosensitivity to the chimeric channels. These experiments strongly support a tether mechanism of mechanogating for the NOMPC channel, providing insights into the basis of mechanosensitivity of mechanotransduction channels.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC4568062 (PMC) (EuropePMC)
Related Publication(s)
Note

The Touching Tail of a Mechanotransduction Channel.
Knecht et al., 2015, Cell 162(6): 1214--1216 [FBrf0230457]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Alleles (9)
    Genes (2)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (3)
    Transgenic Constructs (6)