The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Hugo Bellen at the Baylor College of Medicine. Insertions of constructs from Li et al. (2016) (FBrf0232422): PBac{UAS-Ubr3.L.FLAG}VK00033 is a third chromosome insertion into the PBac{y+-attP-3B}VK00033 target site at 65B2 (R6, 3L:6442676 ), homozygous semi-lethal. PBac{UAS-Ubr3.RINGmut.FLAG}VK00033 is a third chromosome insertion into the PBac{y+-attP-3B}VK00033 target site at 65B2 (R6, 3L:6442676 ), homozygous viable and fertile. The following constructs are unpublished: 1) PBac{UAS-hUBR3.EGFP} carries a human UBR3 with EGFP fused at the C-terminus. PBac{UAS-hUBR3.EGFP}VK00037 is a second chromosome insertion into the PBac{y+-attP-3B}VK00037 target site at 22A3 (R6, 2L:1582820 ), homozygous viable and fertile. PBac{UAS-hUBR3.EGFP}VK00033 is a third chromosome insertion into the PBac{y+-attP-3B}VK00033 target site at 65B2 (R6, 3L:6442676 ), homozygous viable and fertile. 2) PBac{3xFlag.2xHA.Ubr3.18.3} carries a full length genomic fragment of Ubr3 with FLAG and HA tag fused at the N-terminus. PBac{3xFlag.2xHA.Ubr3.18.3}VK00033 is a third chromosome insertion into the PBac{y+-attP-3B}VK00033 target site at 65B2 (R6, 3L:6442676 ), homozygous viable. 3) PBac{Ubr3.18.3.EGFP} carries a full length genomic fragment of Ubr3 with EGFP fused at the C-terminus. PBac{Ubr3.18.3.EGFP}VK00033 is a third chromosome insertion into the PBac{y+-attP-3B}VK00033 target site at 65B2 (R6, 3L:6442676 ), homozygous viable.