FB2026_03 , released September 17, 2026
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Adler, P. (2019.11.26). Adler kkv insertions and alleles. 
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FBrf0244175
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The following information accompanied stocks donated to the Bloomington Stock Center by Paul Adler, University of Virginia. It has been summarized here from a bioRxv preprint (https://doi.org/10.1101/718841), which provided more detailed information.
PBac{UAS-kkv.mNG} is a UAST construct expressing kkv isoform A tagged at the C terminus with mNeonGreen. 
PBac{UAS-kkv.R896K.mNG} is identical to PBac{UAS-kkv.mNG} except the mutation present in kkv1 has been introduced to make a R896K substitution.
PBac{UAS-kkv.mos.mNG} is identical to PBac{UAS-kkv.mNG} except the fly catalytic domain has been mutated so that it is identical to the sequence of the equivalent domain in mosquito (several mosquito orthologs have an identical sequence in this region).
PBac{UAS-kkv.ollas-6xHis} is identical to PBac{UAS-kkv.mNG} except the C terminus is tagged with ollas and 6xHis.
PBac{UAS-kkv.mNG}VK00033, PBac{UAS-kkv.R896K.mNG}VK00033, PBac{UAS-kkv.mos.mNG}VK00033 and PBac{UAS-kkv.ollas-6xHis}VK00033 are insertions into the PBac{y+-attP-3B}VK00033 at 65B2,  3L:6442676  (R6).
TI{TI}kkvmNG was generated by homologous replacement to add mNeonGreen to the kkv isoform A C terminus using the pHD-DsRed vector (Addgene plasmid #51434). It carries a DsRed cassette flanked by loxP sites.
TI{TI}kkvmNG.DsRed- is identical to TI{TI}kkvmNG except the loxP-flanked DsRed cassette has been removed by cre recombinase.
TI{TI}kkvsmGFP-HA is identical to TI{TI}kkvmNG except homologous replacement was used to add smGFP-HA to the kkv isoform A C terminus.
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