FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Reference
Citation
Archambault, V. (2019.11.16). RNAi resistant polo constructs and insertions. 
FlyBase ID
FBrf0244324
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Personal communication to FlyBase
Abstract
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Text of Personal Communication 
The following information accompanied stocks donated to the Bloomington Stock Center by Vince Archambault, Universite de Montreal.
P{UASp-polo-T182A.NLS7A.GFP.RNAi-res} is identical to P{UASp-polo.GFP.RNAi-res} except for sequence changes that substitute seven alanines into the nuclear localization sequence and a non-phosphorylatable alanine for threonine 182.
P{UASp-polo.T182D.NLS7A.GFP.RNAi-res} is identical to P{UASp-polo.GFP.RNAi-res} except for sequence changes that substitute seven alanines into the nuclear localization sequence and a phosphomimetic aspartic acid for threonine 182.
Both constructs were generated in work related to that presented in Karchaner et al. (2017; FBrf0237276).
P{UASp-polo.T182D.NLS7A.GFP.RNAi-res}attP154 and P{UASp-polo-T182A.NLS7A.GFP.RNAi-res}attP154 are insertions into P{CaryP}attP154 at 97D1,  3R:26798991  (R6).
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    English
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    Alleles (2)
    Genes (1)
    Natural transposons (1)
    Insertions (3)
    Experimental Tools (2)
    Transgenic Constructs (2)