The following information accompanied stocks donated to the Bloomington Stock Center by Vince Archambault, Universite de Montreal. Vectors, transgene constructs/insertions and methods for generating Protein A- or GFP-tagged proteins were described in Lipinszki et al. (2014) "Affinity purification of protein complexes from Drosophila embryos in cell cycle studies" Methods Mol. Biol. 1170: 571-588 (FBrf0225277). Using these methods, the following constructs were generated to place the Protein A tag at the C-terminus of the designated protein P{UASp-PrA.GFP} P{UASp-Zw10.PrA} P{UASp-mei-S332.PrA} P{UASp-endos.PrA} P{UASp-barr.PrA} P{UASp-aurB.PrA} P{UASp-elgi.PrA} P{UASp-CycA.PrA} P{UASp-CycB3.PrA} P{UASp-CycB.PrA} and the following constructs were generated to place the Protein A tag at the N-terminus of the designated protein P{UASp-PrA.14-3-3epsilon} P{UASp-PrA.14-3-3zeta} P{UASp-PrA.nudE} P{UASp-PrA.tws} P{UASp-PrA.Skeletor} P{UASp-PrA.Chro} P{UASp-PrA.Lam} (the P{UASp-PrA.tws} construct encodes the long isoform of tws). The P{UASp-GFP.nudE} was generated to place the GFP tag at the N-terminus of the nudE protein. In addition, Archambault and colleagues generated P{UASp-endos.RFP} to place an RFP tag at the C-terminus of the endos protein. The following insertions lie on chromosome 2: P{UASp-endos.RFP}2 P{UASp-PrA.GFP}2 P{UASp-endos.PrA}2 P{UASp-barr.PrA}2 P{UASp-CycA.PrA}2 P{UASp-CycB3.PrA}2 P{UASp-PrA.Lam}2 P{UASp-PrA.14-3-3zeta}2 P{UASp-CycB.PrA}2 P{UASp-PrA.14-3-3epsilon}2 The following insertions lie on chromosome 3: P{UASp-PrA.GFP}3 P{UASp-Zw10.PrA}3 P{UASp-PrA.nudE}3 P{UASp-mei-S332.PrA}3 P{UASp-endos.PrA}3 P{UASp-PrA.Skeletor}3 P{UASp-PrA.Chro}3 P{UASp-barr.PrA}3 P{UASp-aurB.PrA}3 P{UASp-PrA.tws}3 P{UASp-GFP.nudE}3 P{UASp-PrA.Lam}3 P{UASp-elgi.PrA}3 P{UASp-CycA.PrA}3 P{UASp-CycB3.PrA}3 P{UASp-CycB.PrA}3 P{UASp-PrA.14-3-3epsilon}3 P{UASp-PrA.14-3-3zeta}3