FB2026_03 , released September 17, 2026
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Archambault, V. (2019.12.8). Archambault tagged-protein constructs and insertions. 
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FBrf0244325
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Personal communication to FlyBase
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The following information accompanied stocks donated to the Bloomington Stock Center by Vince Archambault, Universite de Montreal.
Vectors, transgene constructs/insertions and methods for generating Protein A- or GFP-tagged proteins were described in Lipinszki et al. (2014) "Affinity purification of protein complexes from Drosophila embryos in cell cycle studies" Methods Mol. Biol. 1170: 571-588 (FBrf0225277). Using these methods, the following constructs were generated to place the Protein A tag at the C-terminus of the designated protein
P{UASp-PrA.GFP}
P{UASp-Zw10.PrA}
P{UASp-mei-S332.PrA}
P{UASp-endos.PrA}
P{UASp-barr.PrA}
P{UASp-aurB.PrA}
P{UASp-elgi.PrA}
P{UASp-CycA.PrA}
P{UASp-CycB3.PrA}
P{UASp-CycB.PrA}
and the following constructs were generated to place the Protein A tag at the N-terminus of the designated protein
P{UASp-PrA.14-3-3epsilon}
P{UASp-PrA.14-3-3zeta}
P{UASp-PrA.nudE}
P{UASp-PrA.tws}
P{UASp-PrA.Skeletor}
P{UASp-PrA.Chro}
P{UASp-PrA.Lam}
(the P{UASp-PrA.tws} construct encodes the long isoform of tws).
The P{UASp-GFP.nudE} was generated to place the GFP tag at the N-terminus of the nudE protein.
In addition, Archambault and colleagues generated P{UASp-endos.RFP} to place an RFP tag at the C-terminus of the endos protein.
The following insertions lie on chromosome 2:
P{UASp-endos.RFP}2
P{UASp-PrA.GFP}2
P{UASp-endos.PrA}2
P{UASp-barr.PrA}2
P{UASp-CycA.PrA}2
P{UASp-CycB3.PrA}2
P{UASp-PrA.Lam}2
P{UASp-PrA.14-3-3zeta}2
P{UASp-CycB.PrA}2
P{UASp-PrA.14-3-3epsilon}2 
The following insertions lie on chromosome 3:
P{UASp-PrA.GFP}3
P{UASp-Zw10.PrA}3
P{UASp-PrA.nudE}3
P{UASp-mei-S332.PrA}3
P{UASp-endos.PrA}3
P{UASp-PrA.Skeletor}3
P{UASp-PrA.Chro}3
P{UASp-barr.PrA}3
P{UASp-aurB.PrA}3
P{UASp-PrA.tws}3
P{UASp-GFP.nudE}3
P{UASp-PrA.Lam}3
P{UASp-elgi.PrA}3
P{UASp-CycA.PrA}3
P{UASp-CycB3.PrA}3
P{UASp-CycB.PrA}3
P{UASp-PrA.14-3-3epsilon}3
P{UASp-PrA.14-3-3zeta}3
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    Alleles (19)
    Genes (17)
    Natural transposons (1)
    Insertions (28)
    Experimental Tools (4)
    Transgenic Constructs (19)