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Citation
Revaitis, N.T., Niepielko, M.G., Marmion, R.A., Klein, E.A., Piccoli, B., Yakoby, N. (2020). Quantitative analyses of EGFR localization and trafficking dynamics in the follicular epithelium.  Development 147(15): dev183210.
FlyBase ID
FBrf0246491
Publication Type
Research paper
Abstract

To bridge the gap between qualitative and quantitative analyses of the epidermal growth factor receptor (EGFR) in tissues, we generated an sfGFP-tagged EGF receptor (EGFR-sfGFP) in Drosophila. The homozygous fly appears similar to wild type with EGFR expression and activation patterns that are consistent with previous reports in the ovary, early embryo, and imaginal discs. Using ELISA, we quantified an average of 1100, 6200 and 2500 receptors per follicle cell (FC) at stages 8/9, 10 and ≥11 of oogenesis, respectively. Interestingly, the spatial localization of the EGFR to the apical side of the FCs at early stages depended on the TGFα-like ligand Gurken. At later stages, EGFR localized to basolateral positions of the FCs. Finally, we followed the endosomal localization of EGFR in the FCs. The EGFR colocalized with the late endosome, but no significant colocalization of the receptor was found with the early endosome. The EGFR-sfGFP fly is an exciting new resource for studying cellular localization and regulation of EGFR in tissues.

PubMed ID
PubMed Central ID
PMC7438018 (PMC) (EuropePMC)
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Personal communication to FlyBase

Location data for grk[null].
Revaitis and Yakoby, 2020.10.27, Location data for grk[null]. [FBrf0247061]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Development
    Title
    Development
    Publication Year
    1987-
    ISBN/ISSN
    0950-1991
    Data From Reference
    Alleles (5)
    Genes (5)
    Insertions (2)
    Experimental Tools (3)
    Transgenic Constructs (4)