The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Maria Landínez-Macías and Olivier Urwyler, University of Zurich.
P{UAS-Ptp69D.UTRs.24xMS2} Expresses Ptp69D with both 5' and 3' UTRs and 24 copies of MS2 protein binding sites under the regulation of UASt.
P{UAS-Kaede.Ptp69D.UTRs} Expresses the Kaede CDS with the Ptp69D 5' and 3' UTRs under the regulation of UASt.
P{UAS-msi.RA.UTRs} Expresses msi RA isoform with both the 5' and 3' UTRs under the regulation of UASt.
P{UAS-Ptp69D.UTRs.24xMS2}attP40 is an insertion at the P{CaryP}attP40 site, 2L, 25C6.
P{UAS-Ptp69D.UTRs.mCherry}attP30 is an insertion at the P{CaryP}attP30 site, 2L, 29C3.
P{UAS-Msi.AdarCD(E488Q)}attP40 is an insertion at the P{CaryP}attP40 site, 2L, 25C6.
P{UAS-Kaede.Ptp69D.UTRs}attP40 is an insertion at the P{CaryP}attP40 site, 2L, 25C6.
P{UAS-msi.RA.UTRs}attP40 is an insertion at the P{CaryP}attP40 site, 2L, 25C6.
These constructs/insertions were made with similar methods described in FBrf0252632, Landínez-Macías, M., Qi, W., Bratus-Neuenschwander, A., Müller, M., Urwyler, O. (2021). The RNA-binding protein Musashi controls axon compartment-specific synaptic connectivity through ptp69D mRNA poly(A)-tailing. Cell Rep. 36(11): 109713.