The wake gene is located at  3R:22630971..22692598  (61.63 Kb) on the (+) strand. We mapped the P{UAS-Mob2.ECFP}wake32099 insertion between  3R:22666897..22666898  on the (+) strand.
There is a mistake in the description of wake15185. It was originally designed two CRISPR target sites to produce wake-Gal4 line, we incorrectly described as the other one. The CRISPR Target Site 'Detailed Information' should be
CRISPR Target Site PAM: ATGGAGCAGGATGCCGCAGCAGG
CRISPR Target Strand Relative to Gene: minus
Cutting Site: 17 bases after ATG of wake-RG
Guide RNA Primers:
Sense oligo 5’-CTTCGATGGAGCAGGATGCCGCAGC
Antisense oligo 5’-AAACGCTGCGGCATCCTGCTCCATC
Upstream Homology Arm: 1024 bp, base -1024~-1 relative to ATG of wake-RG
Forward oligo 5’-TAGAAGTCACCAGCGACAGC
Reverse oligo 5’-GTTGGCTGTGCGAATGCT
Downstream Homology Arm: 1062 bp, base +8~+1069 relative to ATG of wake-RG
Forward oligo 5’-AGGATGCCGCAGCAGGTC
Reverse oligo 5’-GCATCCTGGCCACCAAATG
In wake15185, bases 1-20 from the ATG ( 3R:22652428..22652447 ) have been deleted and replaced by the knock-in-cassette RMCE-Gal4-w with an attP,  Gal4::VP16 , floxed white, and an inverted attP.