Gonzalez, A.C., Abreu, C., Pantano, S., Comini, M., Malacrida, L., Egger, B., Cantera, R., Prieto, D. (2023). A FRET-based cGMP biosensor in Drosophila. MicroPubl Biol 2023(): .
FlyBase ID
FBrf0258331
Publication Type
Research paper
Abstract
CUTie2 is a FRET-based cGMP biosensor tested so far only in cells. To expand its use to multicellular organisms we generated two transgenic Drosophila melanogaster strains that express the biosensor in a tissue-dependent manner. CUTie2 expression and subcellular localization was verified by confocal microscopy. The performance of CUTie2 was analyzed on dissected larval brains by hyperspectral microscopy and flow cytometry. Both approaches confirmed its responsivity, and the latter showed a rapid and reversible change in the fluorescence of the FRET acceptor upon cGMP treatment. This validated reporter system may prove valuable for studying cGMP signaling at organismal level.
