Harris, R., Prasad, I., Weaver, S.E., Abdulrab, A., Abernathy, A.A., Ackerman, A., Acosta, A., Aguirre, A., Al Wakeel, H., Alachkar, F., Alfaro, S., Allen, B., Anderson, A., Araiza, A., Arroyo, K., Arshad, K., Artis, S., Baqa, M.W., Bauer, H.E., Beduhn, P.L., Boylan, G., Brahmbhatt, V., Brock, M., Bryant, R., Byrne, C., Carreno, R., Conteh, A., Cox, K., Daniel, E.M., Drammeh, M., Espino, B., Farmer, N., Fields, A., Flores Santizo, R., Gisele, S., Goolsby, M.I., Grant, E.A., Graves, X., Greenlaw, G., Guelde, L., Hall, J., Hall, J., Harris, Z., Henry, S.M., Hensley, M., Hurd, K., Jolley, M., Kalaj, E.A., Kallan, A., Kaur, R., Key, C., Klesch, M., Kocher, P., Long, A., Lopez, M., Lucas Ii, J.J., Luth, J.X., Marcinkowski, S.D., Martinez, G., Martinez, K., McCray-Brown, C., McDermott, L., McVay, M., Middleton, S., Milla, A., Mobley, J., Moore, R.L., Morris, I., Newman, M.A., Nwoke, I.C., Perez, A., Phillips, K.D., Ramirez, M., Ramos, E., Reda, A., Redmond, C., Rentchler, M., Rider, R.S., Roschi, A., Rouf, S.A., Scott, L., Shah, K., Shin, C.E., Smith, L., Spahiu, A., St Cloud, J., Truitt, I., Tucker, C., Tyler, K.E., Underwood, Z., Vaughn, C., Vazquez, L., Weiss, M., Whitehead, J., Williams, A., Williams, F., Pezdek, M.N., Shah, H.P., Devergne, O., Peister, A., Stamm, J., Kagey, J.D., Merkle, J.A. (2025). Genetic Mapping and Phenotypic Characterization of the Drosophila AIF [e04281] Allele Reveals Mutant Clone Loss in Mosaic Eyes.  MicroPubl Biol 2025(): .

FBrf0264179

Research paper

As part of the Fly-CURE consortium, a mutant allele of Apoptosis inducing factor (AIF) was characterized using complementation mapping, genomic sequencing, and mosaic phenotypic analysis to investigate its role in cell growth control in Drosophila melanogaster . The AIF [e04281] mutation dramatically reduced homozygous mutant clone size and caused morphological defects in genetically mosaic eyes. Sequencing confirmed a transposon insertion that truncates the AIF protein preceding conserved domains essential for mitochondrial function and apoptosis. The observed clone loss indicates a cell-autonomous requirement for AIF and supports the use of AIF [e04281] as a loss-of-function background for genetic modifier screens on chromosome arm 2L.

PMC12720165 (PMC) (EuropePMC)