FB2026_03 , released September 17, 2026
Sequence Feature: Dmel\dsRNA-KK115458
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General Information
Symbol
Dmel\dsRNA-KK115458
Species
D. melanogaster
Feature type
RNAi_reagent
FlyBase ID
FBsf0000195546
Collection
Associated gene
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:21,428,285..21,428,749 [+]

2L:21,472,628..21,473,092 [+]

2L:21,467,585..21,468,049 [+]

2L:21,418,322..21,418,786 [+]

2L:21,423,224..21,423,688 [+]

2L:21,462,542..21,463,006 [+]

2L:21,457,482..21,457,946 [+]

2L:21,452,421..21,452,885 [+]

2L:21,438,380..21,438,844 [+]

2L:21,433,332..21,433,796 [+]

Genomic Maps
Sequence Data
Length
465
Comments

One copy of repeat shown.

(Keleman et al., 2009.8.5)

Sequence

Sequence Downloader
GGTTCCCTCACTGACAGCATGCTTGGCCAACTCTCCAGGCAAAAGCAGGCGAACAGCCGTTTGGATCTCCCGACTGGTGA
TGGTCGAGCGCTTGTTGTAGTGAGCTAGACGAGACGCTTCGGCAGCAATTCGCTCGAAAATATCATTTACAAAGCTGTTC
ATTATGCTCATCGCCTTCGACGAAATTCCGGTGTCAGGATGGACCTGCTTGAGAACCTTGTAAATGTAGATGGCATAGCT
CTCCTTCCTTTTGCGCTTCTTTTTCTTGTCGGTCTTGGTGATGTTCTTCTGAGCCTTGCCAGCCTTCTTGGCTGCCTTTC
CACTAGTTTTCGGAGGCATTGTTCACGTTACTTATATTTTCACAAACACAATTCACTTATCGTAATGTGGGCCCGAACGC
GTTCACGTTTATACTTTTTTTCGAGCAGTCAATTCAGGTCTAAGTCACCCACCCCTAACTGAATG
Associated Information
Experimental Data
Progenitors
PCR template

genomic DNA

(Keleman et al., 2009.8.5)
Primer 1 
GGTTCCCTCACTGACAGCAT
(Keleman et al., 2009.8.5)
Primer 2 
CATTCAGTTAGGGGTGGGTG
(Keleman et al., 2009.8.5)
Comments
Collection Information
Collection: VDRC-KK
Symbol
VDRC-KK
Title
A set of transgenic RNAi constructs for expression of dsRNA under UAS control, VDRC second generation.
Source and Progenitors
Species of derivation
D. melanogaster
Strain of derivation
Vector of progenitor construct
pKC26
Description

Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.

(Keleman et al., 2009.8.5)

NOTE: Dataset members correspond to the amplicon sequence features; these can be used to retrieve associated constructs and stocks.

(FlyBase Curators, 2013)
Additional data
Sample preparation
Collection preparation

The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y+,w3'}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.

(Keleman et al., 2009.8.5)

The host strain used for transgene integration actually has two landing sites: the annotated site in the 5' UTR of tio at R6:2L:22 ,019,296 (P{attP,y+,w3'}VIE-260B), and a previously non-annotated site at R6:2L:9437482 (P{attP,y+,w3'}VIE-260B-2). The previously non-annotated site seems to be the main integration site, occupied in all 39 lines tested. The originally annotated site was occupied in only 9 of 39 lines tested.

(Green et al., 2014)
Mode of assay
Data analysis
Cell lines
Observed in
Not observed in
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
115458
(Keleman et al., 2009.8.5)
dsRNA-KK115458
Secondary FlyBase IDs
    References (2)