FlyBase Insertion Report: Dmel\P{en-lacZ(Xho)}en[Xho25]

General Information

Symbol

Dmel\P{en-lacZ(Xho)}en^Xho25

Species

D. melanogaster

Name

FlyBase ID

FBti0002246

Feature type

transposable_element_insertion_site

Also Known As

en-lacZ, engrailed-lacZ, PlacZ rhx25, en^Xho25-lacZ

Description

Inserted Element

P{en-lacZ(Xho)}

LINE ID

Affected gene(s)

Ecol\lacZ, en

Viability / fertility

Associated allele(s)

Ecol\lacZ^en-Xho25, en^Xho25

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{en-lacZ(Xho)}

Size (Kb)

Component Allele(s)

Ecol\lacZ^en.Xho

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Marchal et al., 2012, Pereanu and Hartenstein, 2006, Urbach et al., 2006, Yasunaga et al., 2006, Schlichting et al., 2005, Dominguez et al., 2004, Aldaz et al., 2003, Janody et al., 2003, Urbach and Technau, 2003, Urbach and Technau, 2003, Urbach et al., 2003, Kopp and Duncan, 2002, Mirth and Akam, 2002, Apidianakis et al., 2001, Hatini and DiNardo, 2001, Janzer and Steinmann-Zwicky, 2001, Keisman et al., 2001, Dahmann and Basler, 2000, Kennerdell and Carthew, 2000, Kojima et al., 2000, Randsholt et al., 2000, Usui and Simpson, 2000, Gritzan et al., 1999, Lawrence et al., 1999, Murphey et al., 1999, Siegler and Jia, 1999, Wilkie et al., 1999, Hartenstein et al., 1998, Maschat et al., 1998, Nassif et al., 1998, Nassif et al., 1998, Rogina et al., 1998, Saget et al., 1998, Schutt et al., 1998, Akimaru et al., 1997, Brizuela and Kennison, 1997, Casares et al., 1997, Chen and Baker, 1997, Hepker et al., 1997, Kopp and Duncan, 1997, Kopp et al., 1997, Rodriguez and Basler, 1997, Struhl et al., 1997, Chen and Struhl, 1996, Diaz et al., 1996, Martin-Bermudo and Brown, 1996, Milan et al., 1996, Minden, 1996, Phillis et al., 1996, Royet and Finkelstein, 1996, Younossi-Hartenstein et al., 1996, Broadus et al., 1995, Dunin-Borkowski et al., 1995, Felsenfeld and Kennison, 1995, Guillen et al., 1995, Kuhn et al., 1995, Mukherjee et al., 1995, Royet and Finkelstein, 1995, Capdevila et al., 1994, Heemskerk and DiNardo, 1994, Couso et al., 1993, Sampedro et al., 1993, Younossi-Hartenstein et al., 1993, Blair, 1992, Eberl et al., 1992, Vincent and O'Farrell, 1992, Bray and Kafatos, 1991, Ingham et al., 1991, Krasnow et al., 1991, Limbourg-Bouchon et al., 1991, Simcox et al., 1991, Finkelstein and Perrimon, 1990, Hama et al., 1990, Phillips et al., 1990, Williams et al., 1990, Ambrosio et al., 1989, Busturia et al., 1989, Perrimon and Smouse, 1989, Simcox et al., 1989, Busturia and Morata, 1988)

(Hama et al., 1990)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^en-Xho25

Phenotypic Class Phenotype Manifest in Detailed Description

en^Xho25

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

P-stage

wing posterior compartment

(Blair, 1992)

adult stage

wing posterior compartment

(Blair, 1992)

adult abdominal segment posterior compartment

(Struhl et al., 1997)

Additional Information

Statement

Reference

Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d3, d5, v4, v5; deuterocerebrum - d5, d9, d13, v8; protocerebrum - ad3-10, av1, cv4, pd5, pd8

(Urbach and Technau, 2003)

Expression of en-lacZ reporter delimits the segmental boundaries in the embryonic procephalic region of the ectoderm and labels a subset of neuroblasts during stages 9-11.

(Urbach et al., 2003)

In third instar larval abdominal segments, expressed in the posterior compartment, in the posterior dorsal histoblast nest, and in a posterior subset of the cells in the ventral histoblast nests. In adult abdominal segments, expressed in the posterior compartment.

(Struhl et al., 1997)

EcollacZ^en-Xho25 transcript was detected in the posterior compartments of imaginal discs, and in ocellus primordia.

(Tashiro et al., 1993)

In pupal wing, pattern slightly different that detected by antibodies against en protein. High level of expression in posterior compartment of the wing; in addition, a narrow region of faintly labelled cells immediately anterior to the posterior compartment, within the anterior compartment and widest at the distal tip of the wing.

(Blair, 1992)

Expression used as a marker for the posterior compartments of discs. The exact pattern was reported elsewhere. (FBrf0051823).

(Simcox et al., 1991)

Marker for

Dd13 neuroblast

(Urbach and Technau, 2003)

Dd5 neuroblast

(Urbach and Technau, 2003)

Dd9 neuroblast

(Urbach and Technau, 2003)

Dv8 neuroblast

(Urbach and Technau, 2003)

Pad13 neuroblast

(Urbach and Technau, 2003)

Pad3 neuroblast

(Urbach and Technau, 2003)

Pad4 neuroblast

(Urbach and Technau, 2003)

Pad5 neuroblast

(Urbach and Technau, 2003)

Pad6 neuroblast

(Urbach and Technau, 2003)

Pad7 neuroblast

(Urbach and Technau, 2003)

Pad8 neuroblast

(Urbach and Technau, 2003)

Pad9 neuroblast

(Urbach and Technau, 2003)

Pav1 neuroblast

(Urbach and Technau, 2003)

Pcv4 neuroblast

(Urbach and Technau, 2003)

Ppd8 neuroblast

(Urbach and Technau, 2003)

Td3 neuroblast

(Urbach and Technau, 2003)

Td5 neuroblast

(Urbach and Technau, 2003)

Tv4 neuroblast

(Urbach and Technau, 2003)

Tv5 neuroblast

(Urbach and Technau, 2003)

imaginal disc posterior compartment

(Simcox et al., 1991)

neuroblast DALv2

(Urbach and Technau, 2003)

Reflects expression of

(Urbach and Technau, 2003, Urbach et al., 2003, Kennerdell and Carthew, 2000, Struhl et al., 1997, Guillen et al., 1995, Blair, 1992)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

5801

P{en-lacZ(Xho)}en^Xho25/CyO

Kyoto

101554

ry¹ P{en-lacZ(Xho)}en^Xho25 / CyO

External Crossreferences and Linkouts ( 0 )

Comments

Homozygotes occasionally survive to adults (~1%).

(Blair, 1992)

Expression of lacZ in en pattern appears to be due to a combination of proximity of insertion to en gene and en regulatory sequences carried on the transposon.

(Busturia and Morata, 1988)

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

En-GAL4

(Hatini and DiNardo, 2001)

En-LacZ

(Hatini and DiNardo, 2001)

En-lacZ

(Urbach, 2007)

En-lacZ Xho-25

(Gritzan et al., 1999)

P[en-lacZ(Xho)]en^Xho25

(Apidianakis et al., 2001)

PlacZ rhx25

(Hartenstein et al., 1998, Nassif et al., 1998)

P{en-lacZ(Xho)}25

(FlyBase, 1992-)

P{en-lacZ(Xho)}en^Xho25

(FlyBase, 1992-, Busturia and Morata, 1988)

P{w^+mC=lacW}en^ryxho25

(Dominguez et al., 2004)

XhoII5

(Kopp and Duncan, 2002)

en-LacZ

(Aldaz et al., 2003)

en-lacZ

(Marchal et al., 2012, Yasunaga et al., 2006, Schlichting et al., 2005, Janody et al., 2003, Urbach and Technau, 2003, Urbach and Technau, 2003, Urbach et al., 2003, Kopp and Duncan, 2002, Apidianakis et al., 2001, Janzer and Steinmann-Zwicky, 2001, Keisman et al., 2001, Kojima et al., 2000, Randsholt et al., 2000, Usui and Simpson, 2000, Murphey et al., 1999)

en-lacZry xho 25

(Guillen et al., 1995)

en.lacZ

(Lawrence et al., 1999)

en^Xho25-lacZ

(Rubio-Ferrera et al., 2023, Domínguez-Cejudo and Casares, 2015)

enZ

(Dahmann and Basler, 2000)

engrailed-lacZ

(Urbach et al., 2006, Urbach and Technau, 2003, Kennerdell and Carthew, 2000)

enlacZ

(Mirth and Akam, 2002)

ryXho25

(Urbach et al., 2006)

ryxho25lacZ

(Pereanu and Hartenstein, 2006)

Name Synonyms

Secondary FlyBase IDs

References (89)

Report Sections

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