FlyBase Insertion Report: Dmel\P{PZ}dally[06464]

General Information

Symbol

Dmel\P{PZ}dally⁰⁶⁴⁶⁴

Species

D. melanogaster

Name

FlyBase ID

FBti0003888

Feature type

transposable_element_insertion_site

Also Known As

dally-lacZ

Description

Inserted Element

P{PZ}

LINE ID

l(3)06464

Affected gene(s)

dally, Ecol\lacZ

Viability / fertility

viable

Associated allele(s)

dally⁰⁶⁴⁶⁴, Ecol\lacZ^dally-06464

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

3L ( 66E1-66E3 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

66E1-66E3 (near gene of known cytology)

Cytological location (reported)

66E1-66E2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

dally

(Liu et al., 2024, Guo and Wang, 2009, Yan and Lin, 2007, Nakato et al., 1995)

Ecol\lacZ

(Guo and Wang, 2009, Crickmore and Mann, 2007, Makhijani et al., 2007, de Navas et al., 2006, Fujise et al., 2003, Tsuda et al., 2001, Tsuda et al., 1999, Vosshall, 1998.8.10, Jackson et al., 1997, Nakato et al., 1995)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

dally⁰⁶⁴⁶⁴

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^dally-06464

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage -- third instar larval stage

eye-antennal disc

(Zhang et al., 2013)

third instar larval stage

larval inner optic anlage

(Nakato et al., 1995)

lamina anlage

(Nakato et al., 1995)

genital disc | ubiquitous | uniform

(Tsuda et al., 1999)

dorsal-ventral compartment boundary of the wing disc | dorsal to

(Makhijani et al., 2007)

dorsal-ventral compartment boundary of the wing disc | ventral to

(Makhijani et al., 2007)

anterior-posterior compartment boundary of the wing disc | anterior to

(Makhijani et al., 2007)

dorsal-ventral compartment boundary of the haltere disc | dorsal to | restricted

Comment: anterior compartment only

(Makhijani et al., 2007)

dorsal-ventral compartment boundary of the haltere disc | restricted | ventral to

Comment: anterior compartment only

(Makhijani et al., 2007)

adult stage

adult optic lobe

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

oogenesis

germarium cap cell

(Guo and Wang, 2009)

Additional Information

Statement

Reference

In both the 2nd and early 3rd instar larval stages, EcollacZ^dally-06464 was ubiquitously expressed in eye-antennal discs, overlapping with cells positive for phosphorylated Stat92E staining.

(Zhang et al., 2013)

EcollacZ^dally-06464 is expressed in cells on either side of the D/V boundary, and anterior to the A/P boundary, in the wing disc. In haltere disc, EcollacZ^dally-06464 expression is repressed in the posterior compartment, and is expressed along the D/V boundary in the anterior compartment; no haltere disc expression is observed along the A/P boundary.

(Makhijani et al., 2007)

In the adult brain, expressed in the central brain and the optic lobe.

(Vosshall, 1998.8.10)

Enhancer trap staining occurs in thelarval brain in a similar pattern to that of EcollacZ^dally-P1.

(Nakato et al., 1995)

Marker for

germarium cap cell

(Guo and Wang, 2009)

Reflects expression of

dally

(Zhang et al., 2013, Guo and Wang, 2009, Makhijani et al., 2007, Tsuda et al., 1999, Nakato et al., 1995)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)06464

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

(FlyBase, 1992-)

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

11685

P{PZ}dally⁰⁶⁴⁶⁴ P{PZ}l(3)87Df⁰⁶⁴⁶⁴ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Kyoto

109041

w^*; P{PZ}ken⁰⁰⁶²⁸ / CyO; P{PZ}dally⁰⁶⁴⁶⁴ / TM3, Ser¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

P{PZ}dally⁰⁶⁴⁶⁴

(Guo and Wang, 2009, Nakato et al., 1995, FlyBase, 1992-)

P{PZ}l(3)06464⁰⁶⁴⁶⁴

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)06464⁰⁶⁴⁶⁴

(FlyBase, 1992-)

dally-lacZ

(Zhang et al., 2013, Guo and Wang, 2009, Yan and Lin, 2007, de Navas et al., 2006)

dally-lacZ^P2

(Crickmore and Mann, 2007)

dally::lacZ

(Tsuda et al., 1999)

dally^P2-lacZ

(Makhijani et al., 2007)

dallyZ

(Zhao et al., 2020)

Name Synonyms

Secondary FlyBase IDs

FBti0000610

References (31)

Report Sections

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