FlyBase Insertion Report: Dmel\P{lacW}shg[k03401]

General Information

Symbol

Dmel\P{lacW}shg^k03401

Species

D. melanogaster

Name

FlyBase ID

FBti0004007

Feature type

transposable_element_insertion_site

Also Known As

shg-lacZ, shg^k03401

Description

Inserted Element

P{lacW}

LINE ID

l(2)k03401

Affected gene(s)

Ecol\lacZ, shg

Viability / fertility

Associated allele(s)

Ecol\lacZ^shg-k03401, shg^k03401

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

2R ( 57B16 )

Sequence Location

2R:21,056,791..21,056,791 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:21,056,791..21,056,791 [-]

(Tepass et al., 1996)

Orientation

Cytological location (computed by FlyBase)

57B16 (inferred by FlyBase from sequence location)

Cytological location (reported)

57B13-57B14 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Genevet et al., 2009, Brown et al., 2006, Jaiswal et al., 2006, Mathews et al., 2006, Becam et al., 2005, Dumstrei et al., 2003, Niewiadomska et al., 1999, Godt and Tepass, 1998, BDGP Project Members, 1994-1999)

shg

(Thuveson et al., 2019, Fernández et al., 2014, Abreu-Blanco et al., 2011, Cobreros-Reguera et al., 2010, Igaki et al., 2006, Igaki et al., 2006, Tepass et al., 1996)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^shg-k03401

Phenotypic Class Phenotype Manifest in Detailed Description

shg^k03401

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)k03401

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

10377

y¹ w^67c23; P{lacW}shg^k03401/CyO

Kyoto

111074

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}shg^k03401 /CyO y⁺

External Crossreferences and Linkouts ( 0 )

Comments

P{lacW}shg^k03401 insertion site reported as behind nucleotide 368 of GB:D28749 in the 5' UTR of shg. This was interpreted by the curator as meaning inserted after base 368. The insertion orientation was not reported.

(Tepass et al., 1996)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

DE-Cad-lacZ

(Jaiswal et al., 2006)

P^lacWshg^k03401

(Genevet et al., 2009)

P{lacW}shg^k03401

(Tepass et al., 1996, FlyBase, 1992-)

hg^k03401

(Igaki et al., 2006)

shg-lacZ

(Hall et al., 2019, Genevet et al., 2009, Brown et al., 2006)

shg-lacZ^k03401

(Enomoto et al., 2021)

shg^P34-1

(Becam et al., 2005)

shg^P341

(Dumstrei et al., 2003)

shg^P34-lacZ

(Dumstrei et al., 2003)

shg^k03401

(Fernández et al., 2014, Abreu-Blanco et al., 2011, Igaki et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (39)

Report Sections

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