FlyBase Insertion Report: Dmel\P{PZ}hry[08247]

General Information

Symbol

Dmel\P{PZ}hry⁰⁸²⁴⁷

Species

D. melanogaster

Name

FlyBase ID

FBti0004820

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)08247

Affected gene(s)

Ecol\lacZ, hry

Viability / fertility

Associated allele(s)

Ecol\lacZ^h-08247, hry⁰⁸²⁴⁷

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 66D10 )

Sequence Location

3L:8,675,823..8,675,823 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:8,675,823..8,675,823 [+]

Orientation

Cytological location (computed by FlyBase)

66D10 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

G00473

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Bessa et al., 2002, Harvie et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

hry

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^h-08247

Phenotypic Class Phenotype Manifest in Detailed Description

hry⁰⁸²⁴⁷

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

ring gland

(Harvie et al., 1998)

embryonic/larval hindgut

(Meister and Braun, 1995.10)

embryonic/larval midgut | restricted

(Meister and Braun, 1995.10)

embryonic/larval salivary gland

(Meister and Braun, 1995.10)

embryonic/larval optic lobe

(Harvie et al., 1998)

Malpighian tubule

(Meister and Braun, 1995.10)

larval ventral nerve cord

(Harvie et al., 1998)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

adult optic lobe

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult, expressed in the central brain, the optic lobe, and the third antennal segment.

(Vosshall, 1998.8.10)

Expression in larval CNS exhibits a punctate pattern.

(Harvie et al., 1998)

In third instar larva, highest level of expression in the hindgut.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)08247

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11730

P{PZ}hry⁰⁸²⁴⁷ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P1730

(Meister and Braun, 1995.10)

P{PZ}h⁰⁸²⁴⁷

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}hry⁰⁸²⁴⁷

P{PZ}l(3)08247⁰⁸²⁴⁷

(FlyBase, 1992-)

P{ry^+t7.2=PZ}h⁰⁸²⁴⁷

(FlyBase, 1992-)

hairy-lacZ

(Bessa et al., 2002)

Name Synonyms

Secondary FlyBase IDs

FBti0000235

References (13)

Report Sections

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